CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023882
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin-binding protein IPP 
Protein Synonyms/Alias
 Intracisternal A particle-promoted polypeptide; IPP; Kelch-like protein 27 
Gene Name
 IPP 
Gene Synonyms/Alias
 KLHL27 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MANEDCPKAADSPFSubiquitination[1]
185LTKDQLIKILRSEELubiquitination[2]
217KDLGKRRKHVVEVLDubiquitination[1]
238LPPQRLLKYIEGVSDubiquitination[1, 2, 3, 4]
257VALQTLLKEYCEVCKubiquitination[1]
264KEYCEVCKSPKENKFubiquitination[1]
270CKSPKENKFCSFLQTubiquitination[1]
279CSFLQTSKVRPRKKAubiquitination[1]
288RPRKKARKYLYAVGGubiquitination[1]
463EVYDPLSKRWSPLPPubiquitination[1, 2, 4]
520WVEVASMKVPRAGMCubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May play a role in organizing the actin cytoskeleton. 
Sequence Annotation
 DOMAIN 37 104 BTB.
 REPEAT 289 343 Kelch 1.
 REPEAT 344 390 Kelch 2.
 REPEAT 391 437 Kelch 3.
 REPEAT 439 485 Kelch 4.
 REPEAT 487 533 Kelch 5.
 REPEAT 535 583 Kelch 6.  
Keyword
 Actin-binding; Alternative splicing; Complete proteome; Cytoplasm; Cytoskeleton; Kelch repeat; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 584 AA 
Protein Sequence
MANEDCPKAA DSPFSSDKHA QLILAQINKM RNGQHFCDVQ LQVGQESFKA HRLVLAASSP 60
YFAALFTGGM KESSKDVVPI LGIEAGIFQI LLDFIYTGIV NIGVNNVQEL IIAADMLQLT 120
EVVHLCCEFL KGQIDPLNCI GIFQFSEQIA CHDLLEFSEN YIHVHFLEVH SGEEFLALTK 180
DQLIKILRSE ELSIEDEYQV FLAAMQWILK DLGKRRKHVV EVLDPIRFPL LPPQRLLKYI 240
EGVSDFNLRV ALQTLLKEYC EVCKSPKENK FCSFLQTSKV RPRKKARKYL YAVGGYTRLQ 300
GGRWSDSRAL SCVERFDTFS QYWTTVSSLH QARSGLGVTV LGGMVYAIGG EKDSMIFDCT 360
ECYDPVTKQW TTVASMNHPR CGLGVCVCYG AIYALGGWVG AEIGNTIERF DPDENKWEVV 420
GNMAVSRYYF GCCEMQGLIY VIGGISNEGI ELRSFEVYDP LSKRWSPLPP MGTRRAYLGV 480
AALNDCIYSV GGWNETQDAL HTVEKYSFEE EKWVEVASMK VPRAGMCVVA VNGLLYVSGG 540
RSSSHDFLAP GTLDSVEVYN PHSDTWTEIG NMITSRCEGG VAVL 584 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; TAS:ProtInc.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0003779; F:actin binding; TAS:ProtInc. 
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR017096; Kelch-like_gigaxonin.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 
PROSITE
 PS50097; BTB 
PRINTS