CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002733
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Integrin beta-1 
Protein Synonyms/Alias
 Fibronectin receptor subunit beta; VLA-4 subunit beta; CD29 
Gene Name
 Itgb1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
202YISTTPAKLRNPCTSubiquitination[1]
774EKEKMNAKWDTGENPacetylation[2]
774EKEKMNAKWDTGENPubiquitination[1]
784TGENPIYKSAVTTVVubiquitination[1]
794VTTVVNPKYEGK***acetylation[3, 4]
794VTTVVNPKYEGK***ubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. 
Sequence Annotation
 DOMAIN 140 378 VWFA.
 REPEAT 466 515 I.
 REPEAT 516 559 II.
 REPEAT 560 598 III.
 REPEAT 599 635 IV.
 REGION 466 635 Cysteine-rich tandem repeats.
 REGION 785 792 Interaction with ITGB1BP1 (By
 METAL 152 152 Magnesium (By similarity).
 METAL 154 154 Calcium 1; via carbonyl oxygen (By
 METAL 156 156 Calcium 1 (By similarity).
 METAL 157 157 Calcium 1 (By similarity).
 METAL 189 189 Calcium 2 (By similarity).
 METAL 244 244 Calcium 2 (By similarity).
 METAL 246 246 Calcium 2 (By similarity).
 METAL 248 248 Calcium 2; via carbonyl oxygen (By
 METAL 249 249 Calcium 2 (By similarity).
 METAL 249 249 Magnesium (By similarity).
 MOD_RES 186 186 Phosphoserine.
 MOD_RES 777 777 Phosphothreonine.
 MOD_RES 783 783 Phosphotyrosine.
 MOD_RES 794 794 N6-acetyllysine (By similarity).
 CARBOHYD 50 50 N-linked (GlcNAc...) (Potential).
 CARBOHYD 94 94 N-linked (GlcNAc...) (Potential).
 CARBOHYD 97 97 N-linked (GlcNAc...) (Potential).
 CARBOHYD 212 212 N-linked (GlcNAc...) (Potential).
 CARBOHYD 269 269 N-linked (GlcNAc...) (Potential).
 CARBOHYD 363 363 N-linked (GlcNAc...).
 CARBOHYD 366 366 N-linked (GlcNAc...); atypical.
 CARBOHYD 376 376 N-linked (GlcNAc...); atypical.
 CARBOHYD 406 406 N-linked (GlcNAc...) (Potential).
 CARBOHYD 417 417 N-linked (GlcNAc...) (Potential).
 CARBOHYD 481 481 N-linked (GlcNAc...) (Potential).
 CARBOHYD 520 520 N-linked (GlcNAc...) (Potential).
 CARBOHYD 584 584 N-linked (GlcNAc...) (Potential).
 CARBOHYD 669 669 N-linked (GlcNAc...).
 DISULFID 27 45 By similarity.
 DISULFID 35 464 By similarity.
 DISULFID 38 75 By similarity.
 DISULFID 48 64 By similarity.
 DISULFID 207 213 By similarity.
 DISULFID 261 301 By similarity.
 DISULFID 401 415 By similarity.
 DISULFID 435 462 By similarity.
 DISULFID 466 691 By similarity.
 DISULFID 477 489 By similarity.
 DISULFID 486 525 By similarity.
 DISULFID 491 500 By similarity.
 DISULFID 502 516 By similarity.
 DISULFID 531 536 By similarity.
 DISULFID 533 568 By similarity.
 DISULFID 538 553 By similarity.
 DISULFID 555 560 By similarity.
 DISULFID 574 579 By similarity.
 DISULFID 576 607 By similarity.
 DISULFID 581 590 By similarity.
 DISULFID 592 599 By similarity.
 DISULFID 613 618 By similarity.
 DISULFID 615 661 By similarity.
 DISULFID 620 630 By similarity.
 DISULFID 633 636 By similarity.
 DISULFID 640 649 By similarity.
 DISULFID 646 723 By similarity.
 DISULFID 665 699 By similarity.  
Keyword
 Acetylation; Cell adhesion; Cell membrane; Cell projection; Complete proteome; Disulfide bond; Glycoprotein; Integrin; Magnesium; Membrane; Metal-binding; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 798 AA 
Protein Sequence
MNLQLVSWIG LISLICSVFG QTDKNRCLKA NAKSCGECIQ AGPNCGWCTN TTFLQEGMPT 60
SARCDDLEAL KKKGCQPSDI ENPRGSQTIK KNKNVTNRSK GMAEKLRPED ITQIQPQQLL 120
LKLRSGEPQK FTLKFKRAED YPIDLYYLMD LSYSMKDDLE NVKSLGTDLM NEMRRITSDF 180
RIGFGSFVEK TVMPYISTTP AKLRNPCTSE QNCTSPFSYK NVLSLTDRGE FFNELVGQQR 240
ISGNLDSPEG GFDAIMQVAV CGSLIGWRNV TRLLVFSTDA GFHFAGDGKL GGIVLPNDGQ 300
CHLENNVYTM SHYYDYPSIA HLVQKLSENN IQTIFAVTEE FQPVYKELKN LIPKSAVGTL 360
SGNSSNVIQL IIDAYNSLSS EVILENSKLP DGVTINYKSY CKNGVNGTGE NGRKCSNISI 420
GDEVQFEISI TANKCPNKES ETIKIKPLGF TEEVEVVLQF ICKCNCQSHG IPASPKCHEG 480
NGTFECGACR CNEGRVGRHC ECSTDEVNSE DMDAYCRKEN SSEICSNNGE CVCGQCVCRK 540
RDNTNEIYSG KFCECDNFNC DRSNGLICGG NGVCRCRVCE CYPNYTGSAC DCSLDTGPCL 600
ASNGQICNGR GICECGACKC TDPKFQGPTC ETCQTCLGVC AEHKECVQCR AFNKGEKKDT 660
CAQECSHFNL TKVESREKLP QPVQVDPVTH CKEKDIDDCW FYFTYSVNGN NEAIVHVVET 720
PDCPTGPDII PIVAGVVAGI VLIGLALLLI WKLLMIIHDR REFAKFEKEK MNAKWDTGEN 780
PIYKSAVTTV VNPKYEGK 798 
Gene Ontology
 GO:0001669; C:acrosomal vesicle; IEA:Compara.
 GO:0034667; C:alpha3-beta1 integrin complex; IEA:Compara.
 GO:0034679; C:alpha9-beta1 integrin complex; IEA:Compara.
 GO:0005604; C:basement membrane; IEA:Compara.
 GO:0009897; C:external side of plasma membrane; IDA:MGI.
 GO:0030175; C:filopodium; IEA:Compara.
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0030056; C:hemidesmosome; IEA:Compara.
 GO:0014704; C:intercalated disc; IDA:MGI.
 GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
 GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
 GO:0045121; C:membrane raft; IEA:Compara.
 GO:0035748; C:myelin sheath abaxonal region; IDA:BHF-UCL.
 GO:0031594; C:neuromuscular junction; IDA:MGI.
 GO:0032587; C:ruffle membrane; IEA:Compara.
 GO:0042383; C:sarcolemma; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0042277; F:peptide binding; IEA:Compara.
 GO:0004872; F:receptor activity; IEA:InterPro.
 GO:0007161; P:calcium-independent cell-matrix adhesion; IEA:Compara.
 GO:0055007; P:cardiac muscle cell differentiation; IMP:MGI.
 GO:0001708; P:cell fate specification; IMP:MGI.
 GO:0002042; P:cell migration involved in sprouting angiogenesis; IMP:MGI.
 GO:0033631; P:cell-cell adhesion mediated by integrin; IEA:Compara.
 GO:0007160; P:cell-matrix adhesion; IGI:MGI.
 GO:0006874; P:cellular calcium ion homeostasis; IEA:Compara.
 GO:0071479; P:cellular response to ionizing radiation; IEA:Compara.
 GO:0071260; P:cellular response to mechanical stimulus; IEA:Compara.
 GO:0071305; P:cellular response to vitamin D; IEA:Compara.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:MGI.
 GO:0008354; P:germ cell migration; IMP:MGI.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0007229; P:integrin-mediated signaling pathway; IEA:UniProtKB-KW.
 GO:0007159; P:leukocyte cell-cell adhesion; IEA:Compara.
 GO:0060135; P:maternal process involved in female pregnancy; IEA:Compara.
 GO:2000811; P:negative regulation of anoikis; IEA:Compara.
 GO:0045596; P:negative regulation of cell differentiation; IMP:MGI.
 GO:0031345; P:negative regulation of cell projection organization; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0045665; P:negative regulation of neuron differentiation; IEA:Compara.
 GO:0031175; P:neuron projection development; IMP:MGI.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0030335; P:positive regulation of cell migration; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IMP:MGI.
 GO:0010811; P:positive regulation of cell-substrate adhesion; IEA:Compara.
 GO:0045807; P:positive regulation of endocytosis; IEA:Compara.
 GO:0043410; P:positive regulation of MAPK cascade; IEA:Compara.
 GO:0045666; P:positive regulation of neuron differentiation; IEA:Compara.
 GO:0010976; P:positive regulation of neuron projection development; IEA:Compara.
 GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IEA:Compara.
 GO:0032594; P:protein transport within lipid bilayer; IEA:Compara.
 GO:0051726; P:regulation of cell cycle; IMP:MGI.
 GO:0008277; P:regulation of G-protein coupled receptor protein signaling pathway; IEA:Compara.
 GO:0014823; P:response to activity; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0034698; P:response to gonadotropin stimulus; IEA:Compara.
 GO:0071559; P:response to transforming growth factor beta stimulus; IEA:Compara.
 GO:0045214; P:sarcomere organization; IMP:MGI.
 GO:0070830; P:tight junction assembly; IEA:Compara.
 GO:0001894; P:tissue homeostasis; IEA:Compara. 
Interpro
 IPR013111; EGF_extracell.
 IPR027071; Integrin_beta-1.
 IPR015812; Integrin_bsu.
 IPR014836; Integrin_bsu_cyt_dom.
 IPR002369; Integrin_bsu_N.
 IPR012896; Integrin_bsu_tail.
 IPR003659; Plexin-like.
 IPR016201; Plexin-like_fold.
 IPR002035; VWF_A. 
Pfam
 PF07974; EGF_2
 PF08725; Integrin_b_cyt
 PF07965; Integrin_B_tail
 PF00362; Integrin_beta 
SMART
 SM00187; INB
 SM00423; PSI
 SM00327; VWA 
PROSITE
 PS00022; EGF_1
 PS00243; INTEGRIN_BETA
 PS50234; VWFA 
PRINTS
 PR01186; INTEGRINB.