UniProt Accession

 SEM5A_RAT; D3ZTD8 

Genbank Protein ID

 CH473992 

Genbank Nucleotide ID

 EDL82657.1 

Protein Name

 Semaphorin-5A 

Protein Synonyms/Alias

 Semaphorin-F; Sema F 

Gene Name

 Sema5a 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
1027	NHINKLDKYDSVEAI	ubiquitination	[1]
1035	YDSVEAIKAFNKNNL	ubiquitination	[1, 2]
1039	EAIKAFNKNNLILEE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis. 

Sequence Annotation

 DOMAIN 35 484 Sema.
 DOMAIN 486 533 PSI.
 DOMAIN 540 593 TSP type-1 1.
 DOMAIN 595 651 TSP type-1 2.
 DOMAIN 653 702 TSP type-1 3.
 DOMAIN 784 839 TSP type-1 4.
 DOMAIN 841 896 TSP type-1 5.
 DOMAIN 897 944 TSP type-1 6.
 CARBOHYD 147 147 N-linked (GlcNAc...) (Potential).
 CARBOHYD 168 168 N-linked (GlcNAc...) (Potential).
 CARBOHYD 227 227 N-linked (GlcNAc...) (Potential).
 CARBOHYD 277 277 N-linked (GlcNAc...) (Potential).
 CARBOHYD 323 323 N-linked (GlcNAc...) (Potential).
 CARBOHYD 367 367 N-linked (GlcNAc...) (Potential).
 CARBOHYD 536 536 N-linked (GlcNAc...) (Potential).
 CARBOHYD 591 591 N-linked (GlcNAc...) (Potential).
 CARBOHYD 717 717 N-linked (GlcNAc...) (Potential).
 CARBOHYD 898 898 N-linked (GlcNAc...) (Potential).
 CARBOHYD 933 933 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1015 1015 N-linked (GlcNAc...) (Potential).
 DISULFID 104 114 By similarity.
 DISULFID 131 140 By similarity.
 DISULFID 254 357 By similarity.
 DISULFID 278 320 By similarity.
 DISULFID 607 644 By similarity.
 DISULFID 611 650 By similarity.
 DISULFID 622 634 By similarity.
 DISULFID 665 696 By similarity.
 DISULFID 669 701 By similarity.
 DISULFID 680 686 By similarity.
 DISULFID 796 833 By similarity.
 DISULFID 800 838 By similarity.
 DISULFID 811 823 By similarity.
 DISULFID 853 890 By similarity.
 DISULFID 857 895 By similarity.
 DISULFID 868 880 By similarity.  

Keyword

 Complete proteome; Developmental protein; Differentiation; Disulfide bond; Glycoprotein; Membrane; Neurogenesis; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1074 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0008046; F:axon guidance receptor activity; IEA:Compara.
 GO:0001569; P:patterning of blood vessels; IEA:Compara. 

Interpro

 IPR003659; Plexin-like.
 IPR016201; Plexin-like_fold.
 IPR027231; Semaphorin.
 IPR001627; Semaphorin/CD100_Ag.
 IPR000884; Thrombospondin_1_rpt.
 IPR015943; WD40/YVTN_repeat-like_dom. 

Pfam

 PF01403; Sema
 PF00090; TSP_1 

SMART

 SM00423; PSI
 SM00630; Sema
 SM00209; TSP1 

PROSITE

 PS51004; SEMA
 PS50092; TSP1 

PRINTS