UniProt Accession

 ARP2_MOUSE; P61161; O15142; Q6PI31 

Genbank Protein ID

 AK032832; AK088216; BC027799; BC047530 

Genbank Nucleotide ID

 BAC28048.1; BAC40216.1; AAH27799.1; AAH47530.1 

Protein Name

 Actin-related protein 2 

Protein Synonyms/Alias

 Actin-like protein 2 

Gene Name

 Actr2 

Gene Synonyms/Alias

 Arp2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
7	*MDSQGRKVVVCDNG	ubiquitination	[1]
46	PIIRSTTKVGNIEIK	acetylation	[2]
46	PIIRSTTKVGNIEIK	succinylation	[2]
46	PIIRSTTKVGNIEIK	ubiquitination	[1]
53	KVGNIEIKDLMVGDE	ubiquitination	[1]
97	DYTFGPEKLNIDTRN	ubiquitination	[1]
118	EPPMNPTKNREKIVE	ubiquitination	[1]
195	DITRYLIKLLLLRGY	acetylation	[3]
219	TVRMIKEKLCYVGYN	acetylation	[3]
219	TVRMIKEKLCYVGYN	ubiquitination	[1]
299	DTRSEFYKHIVLSGG	acetylation	[3, 4, 5]
299	DTRSEFYKHIVLSGG	ubiquitination	[1]
322	SRLERELKQLYLERV	acetylation	[2, 3, 5]
368	LADIMKDKDNFWMTR	acetylation	[2, 3, 5]
368	LADIMKDKDNFWMTR	ubiquitination	[1]
388	KGVRVLEKLGVTVR*	acetylation	[3]
388	KGVRVLEKLGVTVR*	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament (By similarity). 

Sequence Annotation

 NP_BIND 160 162 ATP (By similarity).
 NP_BIND 214 218 ATP (By similarity).
 NP_BIND 305 310 ATP (By similarity).
 MOD_RES 299 299 N6-acetyllysine (By similarity).
 MOD_RES 322 322 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Actin-binding; ATP-binding; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 394 AA 

Protein Sequence

Gene Ontology

 GO:0030478; C:actin cap; IDA:MGI.
 GO:0005885; C:Arp2/3 protein complex; IEA:InterPro.
 GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0030036; P:actin cytoskeleton organization; IMP:MGI.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IEA:InterPro.
 GO:0008356; P:asymmetric cell division; IGI:MGI.
 GO:0016482; P:cytoplasmic transport; IMP:MGI.
 GO:0007163; P:establishment or maintenance of cell polarity; IGI:MGI.
 GO:0051321; P:meiotic cell cycle; IGI:MGI.
 GO:0033206; P:meiotic cytokinesis; IGI:MGI.
 GO:0051653; P:spindle localization; IGI:MGI. 

Interpro

 IPR004000; Actin-related.
 IPR020902; Actin/actin-like_CS.
 IPR027306; Arp2. 

Pfam

 PF00022; Actin 

SMART

 SM00268; ACTIN 

PROSITE

 PS01132; ACTINS_ACT_LIKE 

PRINTS

 PR00190; ACTIN.