UniProt Accession

 PKN2_HUMAN; Q16513; B4DQ21; B4DTP5; B4DVG1; D3DT24; Q08AF4; Q9H1W4 

Genbank Protein ID

 U33052; S75548; AK298595; AK300304; AK301066; AL136381; AC119426; CH471097; CH471097; BC125199 

Genbank Nucleotide ID

 AAC50208.1; AAB33346.1; BAG60783.1; BAG62057.1; BAG62673.1; CAI23271.1; CAI23271.1; EAW73161.1; EAW73164.1; AAI25200.1 

Protein Name

 Serine/threonine-protein kinase N2 

Protein Synonyms/Alias

 PKN gamma; Protein kinase C-like 2; Protein-kinase C-related kinase 2 

Gene Name

 PKN2 

Gene Synonyms/Alias

 PRK2; PRKCL2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
272	SQKLDLLKYSLEQRL	acetylation	[1]
565	ASDSTVTKLDFDLEP	ubiquitination	[2]
784	KIVYRDLKLDNLLLD	ubiquitination	[3]
805	IADFGLCKEGMGYGD	ubiquitination	[4]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication. 

Sequence Annotation

 REPEAT 44 119 REM 1.
 REPEAT 133 213 REM 2.
 REPEAT 214 295 REM 3.
 DOMAIN 330 463 C2.
 DOMAIN 657 916 Protein kinase.
 DOMAIN 917 984 AGC-kinase C-terminal.
 NP_BIND 663 671 ATP (By similarity).
 REGION 382 463 Necessary to rescue apical junction
 REGION 917 977 Necessary for the catalytic activity.
 REGION 978 984 Negatively regulates the responsiveness
 ACT_SITE 782 782 Proton acceptor (By similarity).
 BINDING 686 686 ATP (By similarity).
 MOD_RES 110 110 Phosphoserine.
 MOD_RES 121 121 Phosphothreonine.
 MOD_RES 124 124 Phosphothreonine.
 MOD_RES 302 302 Phosphoserine.
 MOD_RES 306 306 Phosphoserine.
 MOD_RES 360 360 Phosphoserine.
 MOD_RES 362 362 Phosphoserine.
 MOD_RES 367 367 Phosphoserine (By similarity).
 MOD_RES 535 535 Phosphoserine.
 MOD_RES 583 583 Phosphoserine.
 MOD_RES 628 628 Phosphothreonine.
 MOD_RES 631 631 Phosphoserine.
 MOD_RES 816 816 Phosphothreonine; by PDPK1.
 MOD_RES 958 958 Phosphothreonine.  

Keyword

 Alternative splicing; Apoptosis; ATP-binding; Cell adhesion; Cell cycle; Cell division; Cell junction; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 984 AA 

Protein Sequence

Gene Ontology

 GO:0043296; C:apical junction complex; IDA:UniProtKB.
 GO:0032154; C:cleavage furrow; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042826; F:histone deacetylase binding; IDA:UniProtKB.
 GO:0004697; F:protein kinase C activity; IEA:EC.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0043297; P:apical junction assembly; IMP:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0010631; P:epithelial cell migration; IDA:UniProtKB.
 GO:0032467; P:positive regulation of cytokinesis; IMP:UniProtKB.
 GO:0045931; P:positive regulation of mitotic cell cycle; IMP:UniProtKB.
 GO:2000145; P:regulation of cell motility; IMP:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR000961; AGC-kinase_C.
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR011072; HR1_rho-bd.
 IPR011009; Kinase-like_dom.
 IPR017892; Pkinase_C.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF02185; HR1
 PF00069; Pkinase
 PF00433; Pkinase_C 

SMART

 SM00239; C2
 SM00742; Hr1
 SM00133; S_TK_X
 SM00220; S_TKc 

PROSITE

 PS51285; AGC_KINASE_CTER
 PS50004; C2
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS