CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004265
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 POU domain, class 2, transcription factor 1 
Protein Synonyms/Alias
 NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1 
Gene Name
 POU2F1 
Gene Synonyms/Alias
 OCT1; OTF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
284PQSQSTPKRIDTPSLubiquitination[1]
313TFKQRRIKLGFTQGDubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes. 
Sequence Annotation
 DOMAIN 280 354 POU-specific.
 DNA_BIND 379 438 Homeobox.
 MOD_RES 270 270 Phosphothreonine.
 MOD_RES 283 283 Phosphoserine.
 MOD_RES 385 385 Phosphoserine.
 MOD_RES 448 448 Phosphoserine.  
Keyword
 3D-structure; Activator; Alternative splicing; Complete proteome; DNA-binding; Homeobox; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 743 AA 
Protein Sequence
MLDCSDYVLD SRMNNPSETS KPSMESGDGN TGTQTNGLDF QKQPVPVGGA ISTAQAQAFL 60
GHLHQVQLAG TSLQAAAQSL NVQSKSNEES GDSQQPSQPS QQPSVQAAIP QTQLMLAGGQ 120
ITGLTLTPAQ QQLLLQQAQA QAQLLAAAVQ QHSASQQHSA AGATISASAA TPMTQIPLSQ 180
PIQIAQDLQQ LQQLQQQNLN LQQFVLVHPT TNLQPAQFII SQTPQGQQGL LQAQNLLTQL 240
PQQSQANLLQ SQPSITLTSQ PATPTRTIAA TPIQTLPQSQ STPKRIDTPS LEEPSDLEEL 300
EQFAKTFKQR RIKLGFTQGD VGLAMGKLYG NDFSQTTISR FEALNLSFKN MCKLKPLLEK 360
WLNDAENLSS DSSLSSPSAL NSPGIEGLSR RRKKRTSIET NIRVALEKSF LENQKPTSEE 420
ITMIADQLNM EKEVIRVWFC NRRQKEKRIN PPSSGGTSSS PIKAIFPSPT SLVATTPSLV 480
TSSAATTLTV SPVLPLTSAA VTNLSVTGTS DTTSNNTATV ISTAPPASSA VTSPSLSPSP 540
SASASTSEAS SASETSTTQT TSTPLSSPLG TSQVMVTASG LQTAAAAALQ GAAQLPANAS 600
LAAMAAAAGL NPSLMAPSQF AAGGALLSLN PGTLSGALSP ALMSNSTLAT IQALASGGSL 660
PITSLDATGN LVFANAGGAP NIVTAPLFLN PQNLSLLTSN PVSLVSAAAA SAGNSAPVAS 720
LHATSTSAES IQNSLFTVAS ASGAASTTTT ASKAQ 755 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006383; P:transcription from RNA polymerase III promoter; TAS:Reactome. 
Interpro
 IPR017970; Homeobox_CS.
 IPR001356; Homeodomain.
 IPR009057; Homeodomain-like.
 IPR010982; Lambda_DNA-bd_dom.
 IPR013847; POU.
 IPR000327; POU_specific.
 IPR000972; TF_octamer. 
Pfam
 PF00046; Homeobox
 PF00157; Pou 
SMART
 SM00389; HOX
 SM00352; POU 
PROSITE
 PS00027; HOMEOBOX_1
 PS50071; HOMEOBOX_2
 PS00035; POU_1
 PS00465; POU_2
 PS51179; POU_3 
PRINTS
 PR00029; OCTAMER.
 PR00028; POUDOMAIN.