UniProt Accession

 G3P3_YEAST; P00359; D6VUX4; Q6Q5P9 

Genbank Protein ID

 V01300; J01324; X82408; Z72977; AY557831; BK006941 

Genbank Nucleotide ID

 CAA24607.1; AAA88714.1; CAA57803.1; CAA97218.1; AAS56157.1; DAA08285.1 

Protein Name

 Glyceraldehyde-3-phosphate dehydrogenase 3 

Protein Synonyms/Alias

 GAPDH 3 

Gene Name

 TDH3 

Gene Synonyms/Alias

 GPD3; YGR192C; G7576 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
46	DYAAYMFKYDSTHGR	ubiquitination	[1, 2]
63	GEVSHDDKHIIVDGK	acetylation	[3]
63	GEVSHDDKHIIVDGK	ubiquitination	[1, 2]
70	KHIIVDGKKIATYQE	acetylation	[3]
101	IDSTGVFKELDTAQK	acetylation	[3]
108	KELDTAQKHIDAGAK	ubiquitination	[1]
116	HIDAGAKKVVITAPS	acetylation	[4]
116	HIDAGAKKVVITAPS	ubiquitination	[1]
184	HSLTATQKTVDGPSH	acetylation	[3]
184	HSLTATQKTVDGPSH	ubiquitination	[1, 2]
213	PSSTGAAKAVGKVLP	ubiquitination	[1]
217	GAAKAVGKVLPELQG	ubiquitination	[1]
225	VLPELQGKLTGMAFR	ubiquitination	[1]
249	DLTVKLNKETTYDEI	acetylation	[4]
249	DLTVKLNKETTYDEI	ubiquitination	[1]
257	ETTYDEIKKVVKAAA	ubiquitination	[1]
261	DEIKKVVKAAAEGKL	ubiquitination	[1]
267	VKAAAEGKLKGVLGY	ubiquitination	[1]
331	DLVEHVAKA******	ubiquitination	[1]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 

Functional Description

  

Sequence Annotation

 NP_BIND 11 12 NAD (By similarity).
 REGION 149 151 Glyceraldehyde 3-phosphate binding (By
 REGION 209 210 Glyceraldehyde 3-phosphate binding (By
 ACT_SITE 150 150 Nucleophile (By similarity).
 BINDING 33 33 NAD (By similarity).
 BINDING 78 78 NAD; via carbonyl oxygen (By similarity).
 BINDING 180 180 Glyceraldehyde 3-phosphate (By
 BINDING 232 232 Glyceraldehyde 3-phosphate (By
 BINDING 314 314 NAD (By similarity).
 MOD_RES 302 302 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 332 AA 

Protein Sequence

Gene Ontology

 GO:0009277; C:fungal-type cell wall; IDA:SGD.
 GO:0005811; C:lipid particle; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity; IDA:SGD.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006915; P:apoptotic process; IMP:SGD.
 GO:0006094; P:gluconeogenesis; IEP:SGD.
 GO:0006096; P:glycolysis; IEP:SGD.
 GO:0072593; P:reactive oxygen species metabolic process; IMP:SGD. 

Interpro

 IPR020831; GlycerAld/Erythrose_P_DH.
 IPR020830; GlycerAld_3-P_DH_AS.
 IPR020829; GlycerAld_3-P_DH_cat.
 IPR020828; GlycerAld_3-P_DH_NAD(P)-bd.
 IPR006424; Glyceraldehyde-3-P_DH_1.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02800; Gp_dh_C
 PF00044; Gp_dh_N 

SMART

 SM00846; Gp_dh_N 

PROSITE

 PS00071; GAPDH 

PRINTS

 PR00078; G3PDHDRGNASE.