UniProt Accession

 LST2_HUMAN; Q9HCC9; B2RP83; B3KX50; B7Z1Q7; B7Z2G9; B7Z2M2; B7ZB19; E9PB54; E9PB64; E9PG77; Q7Z6J3 

Genbank Protein ID

 AB046863; AK126692; AK293775; AK294710; AK294874; AK316484; AL158068; AL645924; BC032605; BC035793; BC053664; BC137309; BC137310 

Genbank Nucleotide ID

 BAB13469.1; BAG54362.1; BAH11593.1; BAH11855.1; BAH11908.1; BAH14855.1; AAH53664.1; AAI37310.1; AAI37311.1 

Protein Name

 Lateral signaling target protein 2 homolog 

Protein Synonyms/Alias

 hLst2; Zinc finger FYVE domain-containing protein 28 

Gene Name

 ZFYVE28 

Gene Synonyms/Alias

 KIAA1643; LST2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
87	APRDFCVKFPEEIRH	ubiquitination	[1, 2, 3, 4, 5, 6, 7]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Negative regulator of epidermal growth factor receptor (EGFR) signaling. Acts by promoting EGFR degradation in endosomes when not monoubiquitinated. 

Sequence Annotation

 ZN_FING 817 879 FYVE-type.
 MOD_RES 586 586 Phosphoserine; by MAP2K.
 MOD_RES 870 870 Phosphothreonine; by MAP2K.
 CROSSLNK 87 87 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Endosome; Isopeptide bond; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 887 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:UniProtKB.
 GO:0007175; P:negative regulation of epidermal growth factor-activated receptor activity; IMP:UniProtKB. 

Interpro

 IPR000306; Znf_FYVE.
 IPR017455; Znf_FYVE-rel.
 IPR011011; Znf_FYVE_PHD.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF01363; FYVE 

SMART

 SM00064; FYVE 

PROSITE

 PS50178; ZF_FYVE 

PRINTS