CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023901
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 U3 small nucleolar RNA-associated protein 18 homolog 
Protein Synonyms/Alias
 WD repeat-containing protein 50 
Gene Name
 UTP18 
Gene Synonyms/Alias
 WDR50; CDABP0061; CGI-48 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
41GPGGPPQKPAPSSQRubiquitination[1, 2]
172MKNASESKLSKDNLKubiquitination[1]
201PAWAETTKRKTSSDDubiquitination[1]
239PRGILKMKNCQHANAubiquitination[1]
287VDGKTNPKIQSIYLEubiquitination[1, 2, 3, 4, 5, 6]
329VYDMLAGKLIPVHQVubiquitination[1, 3, 5, 6, 7, 8]
395STFSSDSKKVYASSGubiquitination[8]
396TFSSDSKKVYASSGDubiquitination[1]
517FSNFPVIKNKNISHVubiquitination[8]
519NFPVIKNKNISHVHTubiquitination[1, 3, 6]
542YFALGNEKGKALMYRubiquitination[1, 4, 8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 Involved in nucleolar processing of pre-18S ribosomal RNA (By similarity). 
Sequence Annotation
 REPEAT 249 288 WD 1.
 REPEAT 293 333 WD 2.
 REPEAT 339 380 WD 3.
 REPEAT 381 419 WD 4.
 REPEAT 421 462 WD 5.
 REPEAT 471 512 WD 6.
 MOD_RES 121 121 Phosphoserine.
 MOD_RES 124 124 Phosphoserine.
 MOD_RES 204 204 Phosphothreonine.
 MOD_RES 205 205 Phosphoserine.
 MOD_RES 206 206 Phosphoserine.
 MOD_RES 210 210 Phosphoserine.  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; rRNA processing; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 556 AA 
Protein Sequence
MPPERRRRMK LDRRTGAKPK RKPGMRPDWK AGAGPGGPPQ KPAPSSQRKP PARPSAAAAA 60
IAVAAAEEER RLRQRNRLRL EEDKPAVERC LEELVFGDVE NDEDALLRRL RGPRVQEHED 120
SGDSEVENEA KGNFPPQKKP VWVDEEDEDE EMVDMMNNRF RKDMMKNASE SKLSKDNLKK 180
RLKEEFQHAM GGVPAWAETT KRKTSSDDES EEDEDDLLQR TGNFISTSTS LPRGILKMKN 240
CQHANAERPT VARISSVQFH PGAQIVMVAG LDNAVSLFQV DGKTNPKIQS IYLERFPIFK 300
ACFSANGEEV LATSTHSKVL YVYDMLAGKL IPVHQVRGLK EKIVRSFEVS PDGSFLLING 360
IAGYLHLLAM KTKELIGSMK INGRVAASTF SSDSKKVYAS SGDGEVYVWD VNSRKCLNRF 420
VDEGSLYGLS IATSRNGQYV ACGSNCGVVN IYNQDSCLQE TNPKPIKAIM NLVTGVTSLT 480
FNPTTEILAI ASEKMKEAVR LVHLPSCTVF SNFPVIKNKN ISHVHTMDFS PRSGYFALGN 540
EKGKALMYRL HHYSDF 556 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0006364; P:rRNA processing; IEA:UniProtKB-KW. 
Interpro
 IPR024977; Apc4_WD40_dom.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF12894; Apc4_WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS