CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007773
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ran GTPase-activating protein 1 
Protein Synonyms/Alias
 RanGAP1 
Gene Name
 Rangap1 
Gene Synonyms/Alias
 Fug1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
8MASEDIAKLAETLAKacetylation[1]
296RGGLPKLKELNLSFCubiquitination[2]
526LIHMGLLKSEDKIKAacetylation[3]
526LIHMGLLKSEDKIKAsumoylation[4, 5]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Molecular characterization of the SUMO-1 modification of RanGAP1 and its role in nuclear envelope association.
 Mahajan R, Gerace L, Melchior F.
 J Cell Biol. 1998 Jan 26;140(2):259-70. [PMID: 9442102]
 [5] The small ubiquitin-like modifier-1 (SUMO-1) consensus sequence mediates Ubc9 binding and is essential for SUMO-1 modification.
 Sampson DA, Wang M, Matunis MJ.
 J Biol Chem. 2001 Jun 15;276(24):21664-9. [PMID: 11259410
Functional Description
 GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Required for postimplantation development. 
Sequence Annotation
 REPEAT 48 71 LRR 1.
 REPEAT 111 134 LRR 2.
 REPEAT 207 230 LRR 3.
 REPEAT 235 258 LRR 4.
 REPEAT 292 315 LRR 5.
 REPEAT 320 343 LRR 6.
 MOTIF 525 528 SUMO conjugation (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 358 358 Phosphoserine (By similarity).
 MOD_RES 411 411 Phosphothreonine; by CDK2 (By
 MOD_RES 430 430 Phosphoserine (By similarity).
 MOD_RES 437 437 Phosphoserine (By similarity).
 MOD_RES 438 438 Phosphothreonine (By similarity).
 MOD_RES 444 444 Phosphoserine.
 MOD_RES 526 526 N6-acetyllysine; alternate (By
 CROSSLNK 8 8 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 526 526 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; GTPase activation; Isopeptide bond; Kinetochore; Leucine-rich repeat; Membrane; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 589 AA 
Protein Sequence
MASEDIAKLA ETLAKTQVAG GQLSFKGKGL KLNTAEDAKD VIKEIEEFDG LEALRLEGNT 60
VGVEAARVIA KALEKKSELK RCHWSDMFTG RLRSEIPPAL ISLGEGLITA GAQLVELDLS 120
DNAFGPDGVR GFEALLKSPA CFTLQELKLN NCGMGIGGGK ILAAALTECH RKSSAQGKPL 180
ALKVFVAGRN RLENDGATAL AEAFGIIGTL EEVHMPQNGI NHPGVTALAQ AFAINPLLRV 240
INLNDNTFTE KGGVAMAETL KTLRQVEVIN FGDCLVRSKG AVAIADAVRG GLPKLKELNL 300
SFCEIKRDAA LVVAEAVADK AELEKLDLNG NALGEEGCEQ LQEVMDSFNM AKVLASLSDD 360
EGEDEDEEEE GEEDDEEEED EEDEEDDDEE EEEQEEEEEP PQRGSGEEPA TPSRKILDPN 420
SGEPAPVLSS PTPTDLSTFL SFPSPEKLLR LGPKVSVLIV QQTDTSDPEK VVSAFLKVAS 480
VFRDDASVKT AVLDAIDALM KKAFSCSSFN SNTFLTRLLI HMGLLKSEDK IKAIPSLHGP 540
LMVLNHVVRQ DYFPKALAPL LLAFVTKPNG ALETCSFARH NLLQTLYNI 589 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:MGI.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0005098; F:Ran GTPase activator activity; IEA:InterPro.
 GO:0046826; P:negative regulation of protein export from nucleus; IEA:Compara.
 GO:0032853; P:positive regulation of Ran GTPase activity; IEA:GOC.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR009109; Ran_GTPase_activating_1_C.
 IPR027038; RanGap. 
Pfam
 PF07834; RanGAP1_C 
SMART
  
PROSITE
  
PRINTS