UniProt Accession

 CAN2_HUMAN; P17655; A6NDG7; B7ZA96; E7ES58; Q16738; Q6PJT3; Q8WU26; Q9HBB1 

Genbank Protein ID

 M23254; AF261089; AK316211; AY835586; AC096542; AC099065; BC007686; BC011828; BC021303; J04700 

Genbank Nucleotide ID

 AAA35645.1; AAF99682.1; BAH14582.1; AAV80421.1; AAH07686.1; AAH11828.1; AAH21303.1; AAA52760.1 

Protein Name

 Calpain-2 catalytic subunit 

Protein Synonyms/Alias

 Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain; M-calpain 

Gene Name

 CAPN2 

Gene Synonyms/Alias

 CANPL2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
182	ITFQKLVKGHAYSVT	ubiquitination	[1]
398	REVLNRFKLPPGEYI	ubiquitination	[1]
596	VRLETLFKIFKQLDP	ubiquitination	[1]

Reference

 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. 

Sequence Annotation

 DOMAIN 45 344 Calpain catalytic.
 DOMAIN 572 605 EF-hand 1.
 DOMAIN 602 637 EF-hand 2.
 DOMAIN 667 700 EF-hand 3.
 REGION 345 514 Domain III.
 REGION 515 529 Linker.
 REGION 530 700 Domain IV.
 ACT_SITE 105 105 By similarity.
 ACT_SITE 262 262 By similarity.
 ACT_SITE 286 286 By similarity.
 METAL 89 89 Calcium 3; via carbonyl oxygen (By
 METAL 91 91 Calcium 3; via carbonyl oxygen (By
 METAL 96 96 Calcium 3 (By similarity).
 METAL 175 175 Calcium 3 (By similarity).
 METAL 229 229 Calcium 2 (By similarity).
 METAL 230 230 Calcium 2 (By similarity).
 METAL 292 292 Calcium 4 (By similarity).
 METAL 299 299 Calcium 4 (By similarity).
 METAL 323 323 Calcium 4; via carbonyl oxygen (By
 METAL 542 542 Calcium 5; via carbonyl oxygen (By
 METAL 545 545 Calcium 5 (By similarity).
 METAL 547 547 Calcium 5; via carbonyl oxygen (By
 METAL 552 552 Calcium 5 (By similarity).
 METAL 585 585 Calcium 6 (By similarity).
 METAL 587 587 Calcium 6 (By similarity).
 METAL 589 589 Calcium 6; via carbonyl oxygen (By
 METAL 591 591 Calcium 6; via carbonyl oxygen (By
 METAL 596 596 Calcium 6 (By similarity).
 METAL 615 615 Calcium 7 (By similarity).
 METAL 617 617 Calcium 7 (By similarity).
 METAL 619 619 Calcium 7; via carbonyl oxygen (By
 METAL 621 621 Calcium 7; via carbonyl oxygen (By
 METAL 626 626 Calcium 7 (By similarity).
 METAL 658 658 Calcium 1 (By similarity).
 METAL 661 661 Calcium 1 (By similarity).  

Keyword

 3D-structure; Alternative splicing; Calcium; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Membrane; Metal-binding; Polymorphism; Protease; Reference proteome; Repeat; Thiol protease. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 700 AA 

Protein Sequence

Gene Ontology

 GO:0000785; C:chromatin; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:Compara.
 GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:Compara.
 GO:0008234; F:cysteine-type peptidase activity; TAS:ProtInc.
 GO:0001824; P:blastocyst development; IEA:Compara.
 GO:0007520; P:myoblast fusion; IEA:Compara.
 GO:0016540; P:protein autoprocessing; IEA:Compara.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0001666; P:response to hypoxia; IEA:Compara. 

Interpro

 IPR022684; Calpain_cysteine_protease.
 IPR022682; Calpain_domain_III.
 IPR022683; Calpain_III.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000169; Pept_cys_AS.
 IPR001300; Peptidase_C2_calpain_cat. 

Pfam

 PF01067; Calpain_III
 PF00648; Peptidase_C2 

SMART

 SM00720; calpain_III
 SM00230; CysPc
 SM00054; EFh 

PROSITE

 PS50203; CALPAIN_CAT
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS00640; THIOL_PROTEASE_ASN
 PS00139; THIOL_PROTEASE_CYS
 PS00639; THIOL_PROTEASE_HIS 

PRINTS

 PR00704; CALPAIN.