| Tag | Content |
|---|
| CPLM ID | CPLM-003543 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Methylated-DNA--protein-cysteine methyltransferase |
Protein Synonyms/Alias | 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase |
Gene Name | ogt |
Gene Synonyms/Alias | b1335; JW1329 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 65 | NPGGLSDKLREYFAG | acetylation | [1] | | 95 | PFQREVWKTLRTIPC | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine. |
Sequence Annotation | ACT_SITE 139 139 Nucleophile; methyl group acceptor |
Keyword | Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair; Methyltransferase; Reference proteome; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 171 AA |
Protein Sequence | MLRLLEEKIA TPLGPLWVIC DEQFRLRAVE WEEYSERMVQ LLDIHYRKEG YERISATNPG 60
GLSDKLREYF AGNLSIIDTL PTATGGTPFQ REVWKTLRTI PCGQVMHYGQ LAEQLGRPGA 120
ARAVGAANGS NPISIVVPCH RVIGRNGTMT GYAGGVQRKE WLLRHEGYLL L 171 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0003908; F:methylated-DNA-[protein]-cysteine S-methyltransferase activity; IEA:HAMAP. GO:0006307; P:DNA dealkylation involved in DNA repair; IEA:HAMAP. |
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