CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031518
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Neuroepithelial cell-transforming gene 1 protein 
Protein Synonyms/Alias
 cDNA FLJ53956, highly similar to Neuroepithelial cell-transforming gene 1 protein 
Gene Name
 NET1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
13QDLIEDLKLARKAYHubiquitination[1, 2, 3, 4]
17EDLKLARKAYHDPMLubiquitination[4]
60TRIGEATKPDGTVEQubiquitination[4]
93CSNQLAAKALLDQKKubiquitination[3, 4]
99AKALLDQKKQDPRVQubiquitination[4]
120LESPFSRKLDLWSFLubiquitination[4]
136IPRSRLVKYPLLLKEubiquitination[4]
176LSDINLKKGESECQYubiquitination[4]
187ECQYYIDKLEYLDEKubiquitination[4]
204DPRIEASKVLLCHGEubiquitination[4, 5]
308QANDVFHKQQWFNCIubiquitination[4]
386MQMAEDSKSLKTHQTubiquitination[4]
389AEDSKSLKTHQTQPGubiquitination[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 415 AA 
Protein Sequence
MSRGEQDLIE DLKLARKAYH DPMLKLSIMS EEELTHIFGD LDSYIPLHED LLTRIGEATK 60
PDGTVEQIGH ILVSWLPRLN AYRGYCSNQL AAKALLDQKK QDPRVQDFLQ RCLESPFSRK 120
LDLWSFLDIP RSRLVKYPLL LKEILKHTPK EHPDVQLLED AILIIQGVLS DINLKKGESE 180
CQYYIDKLEY LDEKQRDPRI EASKVLLCHG ELRSKSGHKL YIFLFQDILV LTRPVTRNER 240
HSYQVYRQPI PVQELVLEDL QDGDVRMGGS FRGAFSNSEK AKNIFRIRFH DPSPAQSHTL 300
QANDVFHKQQ WFNCIRAAIA PFQSAGSPPE LQGLPELHEE CEGNHPSARK LTAQRRASTV 360
SSVTQVEVDE NAYRCGSGMQ MAEDSKSLKT HQTQPGIRRA RDKALSGGKR KETLV 415 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro. 
Interpro
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR015721; RhoGEF-like. 
Pfam
 PF00169; PH
 PF00621; RhoGEF 
SMART
 SM00233; PH
 SM00325; RhoGEF 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN 
PRINTS