CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013194
UniProt Accession
Genbank Protein ID
 D45408 
Genbank Nucleotide ID
Protein Name
 Elongation factor 1-alpha 
Protein Synonyms/Alias
 EF-1-alpha 
Gene Name
 EF1A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Zea mays (Maize) 
NCBI Taxa ID
 4577 
Lysine Modification
Position
Peptide
Type
References
36YKLGGIDKRVIERFEmethylation[1]
79TIDIALWKFETTKYYmethylation[1]
187KVGYNPDKIHFVPISmethylation[1]
227LDLINEPKRPSDKPLmethylation[1]
306NVKNVAVKDLKRGYVmethylation[1]
396MVKMVPTKPMVVETFmethylation[1]
Reference
 [1] eEF1A isoforms change in abundance and actin-binding activity during maize endosperm development.
 Lopez-Valenzuela JA, Gibbon BC, Hughes PA, Dreher TW, Larkins BA.
 Plant Physiol. 2003 Nov;133(3):1285-95. [PMID: 14526107] 
Functional Description
 This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. 
Sequence Annotation
 NP_BIND 14 21 GTP (By similarity).
 NP_BIND 91 95 GTP (By similarity).
 NP_BIND 153 156 GTP (By similarity).
 MOD_RES 289 289 5-glutamyl glycerylphosphorylethanolamine
 MOD_RES 362 362 5-glutamyl glycerylphosphorylethanolamine  
Keyword
 Cytoplasm; Elongation factor; GTP-binding; Nucleotide-binding; Phosphoprotein; Protein biosynthesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 447 AA 
Protein Sequence
MGKEKTHINI VVIGHVDSGK STTTGHLIYK LGGIDKRVIE RFEKEAAEMN KRSFKYAWVL 60
DKLKAERERG ITIDIALWKF ETTKYYCTVI DAPGHRDFIK NMITGTSQAD CAVLIIDSTT 120
GGFEAGISKD GQTREHALLA FTLGVKQMIC CCNKMDATTP KYSKARYDEI VKEVSSYLKK 180
VGYNPDKIHF VPISGFEGDN MIERSTNLDW YKGPTLLEAL DLINEPKRPS DKPLRLPLQD 240
VYKIGGIGTV PVGRVETGVI KPGMVVTFGP TGLTTEVKSV EMHHEALQEA LPGDNVGFNV 300
KNVAVKDLKR GYVASGSKDD PAKEAASFTS QVIIMNHPGQ IGNGYAPVLD CHTSHIAVKF 360
AELVTKIDRR SGKELEKEPK FLKNGDAGMV KMVPTKPMVV ETFSQYPPLG RFAVRDMRQT 420
VAVGVIKSVE KKDPTGAKVT KAAAKKK 447 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
 GO:0006184; P:GTP catabolic process; IEA:GOC. 
Interpro
 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR009001; Transl_elong_EF1A/Init_IF2_C.
 IPR004539; Transl_elong_EF1A_euk/arc.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR004160; Transl_elong_EFTu/EF1A_C.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF03143; GTP_EFTU_D3 
SMART
  
PROSITE
 PS00301; EFACTOR_GTP 
PRINTS
 PR00315; ELONGATNFCT.