UniProt Accession

 UBXN1_HUMAN; Q04323; Q9BV93; Q9BVV5 

Genbank Protein ID

 M68864; BC000902; BC001372; BC040129 

Genbank Nucleotide ID

 AAA36396.1; AAH00902.1; AAH01372.1; AAH40129.1 

Protein Name

 UBX domain-containing protein 1 

Protein Synonyms/Alias

 SAPK substrate protein 1; UBA/UBX 33.3 kDa protein 

Gene Name

 UBXN1 

Gene Synonyms/Alias

 SAKS1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
105	MLELVAQKQREREER	ubiquitination	[1, 2]
154	AEERRREKAEELAAR	ubiquitination	[2]
178	DKAERAKKYGGSVGS	ubiquitination	[2]
208	PSQEPPTKREYDQCR	ubiquitination	[1]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Ubiquitin-binding protein that interacts with the BRCA1- BARD1 heterodimer, and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1, leads to inhibit the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. 

Sequence Annotation

 DOMAIN 1 42 UBA.
 DOMAIN 211 293 UBX.
 REGION 43 297 Interaction with BRCA1.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 199 199 Phosphoserine.
 MOD_RES 200 200 Phosphoserine; by MAPK12.
 MOD_RES 270 270 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 297 AA 

Protein Sequence

Gene Ontology

 GO:0034098; C:Cdc48p-Npl4p-Ufd1p AAA ATPase complex; IDA:BHF-UCL.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0071796; F:K6-linked polyubiquitin binding; IDA:UniProtKB.
 GO:0031625; F:ubiquitin protein ligase binding; IDA:BHF-UCL.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0031397; P:negative regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; NAS:UniProtKB. 

Interpro

 IPR009060; UBA-like.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk.
 IPR001012; UBX. 

Pfam

 PF00627; UBA
 PF00789; UBX 

SMART

 SM00165; UBA
 SM00166; UBX 

PROSITE

 PS50030; UBA
 PS50033; UBX 

PRINTS