CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007636
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Carnitine O-acetyltransferase 
Protein Synonyms/Alias
 Carnitine acetylase; Carnitine acetyltransferase; CAT; CrAT 
Gene Name
 CRAT 
Gene Synonyms/Alias
 CAT1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
261NHRNSWAKAYNTLIKacetylation[1]
268KAYNTLIKDKVNRDSacetylation[1]
609RLAHYLEKALLDMRAacetylation[2]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. 
Sequence Annotation
 REGION 418 430 Coenzyme A binding (By similarity).
 MOTIF 624 626 Microbody targeting signal (Potential).
 ACT_SITE 343 343 Proton acceptor (Probable).
 BINDING 452 452 Carnitine.
 BINDING 454 454 Carnitine.
 BINDING 465 465 Carnitine.
 MOD_RES 261 261 N6-acetyllysine.
 MOD_RES 268 268 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Acyltransferase; Alternative splicing; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Fatty acid metabolism; Lipid metabolism; Membrane; Mitochondrion; Mitochondrion inner membrane; Peroxisome; Polymorphism; Reference proteome; Transferase; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 626 AA 
Protein Sequence
MLAFAARTVV KPLGFLKPFS LMKASSRFKA HQDALPRLPV PPLQQSLDHY LKALQPIVSE 60
EEWAHTKQLV DEFQASGGVG ERLQKGLERR ARKTENWLSE WWLKTAYLQY RQPVVIYSSP 120
GVMLPKQDFV DLQGQLRFAA KLIEGVLDFK VMIDNETLPV EYLGGKPLCM NQYYQILSSC 180
RVPGPKQDTV SNFSKTKKPP THITVVHNYQ FFELDVYHSD GTPLTADQIF VQLEKIWNSS 240
LQTNKEPVGI LTSNHRNSWA KAYNTLIKDK VNRDSVRSIQ KSIFTVCLDA TMPRVSEDVY 300
RSHVAGQMLH GGGSRLNSGN RWFDKTLQFI VAEDGSCGLV YEHAAAEGPP IVTLLDYVIE 360
YTKKPELVRS PLVPLPMPKK LRFNITPEIK SDIEKAKQNL SIMIQDLDIT VMVFHHFGKD 420
FPKSEKLSPD AFIQMALQLA YYRIYGQACA TYESASLRMF HLGRTDTIRS ASMDSLTFVK 480
AMDDSSVTEH QKVELLRKAV QAHRGYTDRA IRGEAFDRHL LGLKLQAIED LVSMPDIFMD 540
TSYAIAMHFH LSTSQVPAKT DCVMFFGPVV PDGYGVCYNP MEAHINFSLS AYNSCAETNA 600
ARLAHYLEKA LLDMRALLQS HPRAKL 626 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005782; C:peroxisomal matrix; TAS:Reactome.
 GO:0004092; F:carnitine O-acetyltransferase activity; IDA:UniProtKB.
 GO:0019254; P:carnitine metabolic process, CoA-linked; IDA:UniProtKB.
 GO:0033540; P:fatty acid beta-oxidation using acyl-CoA oxidase; TAS:Reactome.
 GO:0006810; P:transport; IEA:UniProtKB-KW. 
Interpro
 IPR000542; Carn_acyl_trans. 
Pfam
 PF00755; Carn_acyltransf 
SMART
  
PROSITE
 PS00439; ACYLTRANSF_C_1
 PS00440; ACYLTRANSF_C_2 
PRINTS