CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010993
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Importin subunit alpha 
Protein Synonyms/Alias
 Karyopherin subunit alpha; Karyopherin-60; Serine-rich RNA polymerase I suppressor protein 
Gene Name
 SRP1 
Gene Synonyms/Alias
 KAP60; YNL189W; N1606 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
11GTDSSTSKFVPEYRRacetylation[1]
398LLEVAEYKTKKEACWacetylation[1]
398LLEVAEYKTKKEACWubiquitination[2]
496CQQNENDKIYEKAYKubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity). 
Sequence Annotation
 DOMAIN 1 65 IBB.
 REPEAT 89 122 ARM 1; truncated.
 REPEAT 123 162 ARM 2.
 REPEAT 163 204 ARM 3.
 REPEAT 205 251 ARM 4.
 REPEAT 252 288 ARM 5.
 REPEAT 289 330 ARM 6.
 REPEAT 331 372 ARM 7.
 REPEAT 373 417 ARM 8.
 REPEAT 418 471 ARM 9.
 REPEAT 472 508 ARM 10; atypical.
 REGION 209 335 NLS binding site 1.
 REGION 419 505 NLS binding site 2.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 542 AA 
Protein Sequence
MDNGTDSSTS KFVPEYRRTN FKNKGRFSAD ELRRRRDTQQ VELRKAKRDE ALAKRRNFIP 60
PTDGADSDEE DESSVSADQQ FYSQLQQELP QMTQQLNSDD MQEQLSATVK FRQILSREHR 120
PPIDVVIQAG VVPRLVEFMR ENQPEMLQLE AAWALTNIAS GTSAQTKVVV DADAVPLFIQ 180
LLYTGSVEVK EQAIWALGNV AGDSTDYRDY VLQCNAMEPI LGLFNSNKPS LIRTATWTLS 240
NLCRGKKPQP DWSVVSQALP TLAKLIYSMD TETLVDACWA ISYLSDGPQE AIQAVIDVRI 300
PKRLVELLSH ESTLVQTPAL RAVGNIVTGN DLQTQVVINA GVLPALRLLL SSPKENIKKE 360
ACWTISNITA GNTEQIQAVI DANLIPPLVK LLEVAEYKTK KEACWAISNA SSGGLQRPDI 420
IRYLVSQGCI KPLCDLLEIA DNRIIEVTLD ALENILKMGE ADKEARGLNI NENADFIEKA 480
GGMEKIFNCQ QNENDKIYEK AYKIIETYFG EEEDAVDETM APQNAGNTFG FGSNVNQQFN 540
FN 542 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008565; F:protein transporter activity; IDA:SGD.
 GO:0006606; P:protein import into nucleus; IMP:SGD.
 GO:0006612; P:protein targeting to membrane; IMP:SGD. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR002652; Importin-a_IBB.
 IPR024931; Importing_su_alpha. 
Pfam
 PF00514; Arm
 PF01749; IBB 
SMART
 SM00185; ARM 
PROSITE
 PS50176; ARM_REPEAT
 PS51214; IBB 
PRINTS