CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008428
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chloride transport protein 6 
Protein Synonyms/Alias
 Chloride channel protein 6; ClC-6 
Gene Name
 CLCN6 
Gene Synonyms/Alias
 KIAA0046 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
46EDEILPRKDYESLDYubiquitination[1]
224FQSISLRKIQFNFPYubiquitination[1]
648GNEKEFMKGNQLISNubiquitination[1, 2]
679RKRSQSMKSYPSSELubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Chloride transport protein, initially identified as voltage-gated chloride channel. The presence of the conserved gating glutamate residues suggests that is functions as antiporter. 
Sequence Annotation
 DOMAIN 605 662 CBS 1.
 DOMAIN 807 868 CBS 2.
 NP_BIND 630 632 ATP (By similarity).
 NP_BIND 849 852 ATP (By similarity).
 MOTIF 156 160 Selectivity filter part_1 (By
 MOTIF 198 202 Selectivity filter part_2 (By
 MOTIF 487 491 Selectivity filter part_3 (By
 BINDING 157 157 Chloride (By similarity).
 BINDING 489 489 Chloride; via amide nitrogen (By
 BINDING 576 576 Chloride (By similarity).
 CARBOHYD 410 410 N-linked (GlcNAc...).
 CARBOHYD 422 422 N-linked (GlcNAc...).
 CARBOHYD 432 432 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Antiport; ATP-binding; CBS domain; Chloride; Complete proteome; Endosome; Glycoprotein; Ion transport; Membrane; Nucleotide-binding; Polymorphism; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 869 AA 
Protein Sequence
MAGCRGSLCC CCRWCCCCGE RETRTPEELT ILGETQEEED EILPRKDYES LDYDRCINDP 60
YLEVLETMDN KKGRRYEAVK WMVVFAIGVC TGLVGLFVDF FVRLFTQLKF GVVQTSVEEC 120
SQKGCLALSL LELLGFNLTF VFLASLLVLI EPVAAGSGIP EVKCYLNGVK VPGIVRLRTL 180
LCKVLGVLFS VAGGLFVEKE GPMIHSGSVV GAGLPQFQSI SLRKIQFNFP YFRSDRDKRD 240
FVSAGAAAGV AAAFGAPIGG TLFSLEEGSS FWNQGLTWKV LFCSMSATFT LNFFRSGIQF 300
GSWGSFQLPG LLNFGEFKCS DSDKKCHLWT AMDLGFFVVM GVIGGLLGAT FNCLNKRLAK 360
YRMRNVHPKP KLVRVLESLL VSLVTTVVVF VASMVLGECR QMSSSSQIGN DSFQLQVTED 420
VNSSIKTFFC PNDTYNDMAT LFFNPQESAI LQLFHQDGTF SPVTLALFFV LYFLLACWTY 480
GISVPSGLFV PSLLCGAAFG RLVANVLKSY IGLGHIYSGT FALIGAAAFL GGVVRMTISL 540
TVILIESTNE ITYGLPIMVT LMVAKWTGDF FNKGIYDIHV GLRGVPLLEW ETEVEMDKLR 600
ASDIMEPNLT YVYPHTRIQS LVSILRTTVH HAFPVVTENR GNEKEFMKGN QLISNNIKFK 660
KSSILTRAGE QRKRSQSMKS YPSSELRNMC DEHIASEEPA EKEDLLQQML ERRYTPYPNL 720
YPDQSPSEDW TMEERFRPLT FHGLILRSQL VTLLVRGVCY SESQSSASQP RLSYAEMAED 780
YPRYPDIHDL DLTLLNPRMI VDVTPYMNPS PFTVSPNTHV SQVFNLFRTM GLRHLPVVNA 840
VGEVSEAPAL PPPLREDPLA RCCLCTQASH QKRRHPTRRG ECGPTLALNP ARLPCTRDPF 900
PCLPADGTSV PLAVLSSQSR ASTRLCLPPE MLLFTPYHWC SLVLHLRRDL RIR 953 
Gene Ontology
 GO:0010008; C:endosome membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005247; F:voltage-gated chloride channel activity; NAS:UniProtKB.
 GO:0006884; P:cell volume homeostasis; NAS:UniProtKB.
 GO:0007165; P:signal transduction; NAS:UniProtKB. 
Interpro
 IPR014743; Cl-channel_core.
 IPR001807; Cl-channel_volt-gated.
 IPR002248; Cl_channel-6.
 IPR000644; Cysta_beta_synth_core. 
Pfam
 PF00571; CBS
 PF00654; Voltage_CLC 
SMART
 SM00116; CBS 
PROSITE
 PS51371; CBS 
PRINTS
 PR00762; CLCHANNEL.
 PR01117; CLCHANNEL6.