CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020011
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histidine-rich glycoprotein 
Protein Synonyms/Alias
 Histidine-proline-rich glycoprotein; HPRG; Histidine-rich glycoprotein 1; HRG1 
Gene Name
 Hrg 
Gene Synonyms/Alias
 Hrg1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
254LKNRSDMKPHRGHEHacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Inhibits rosette formation. Acts as an adapter protein and implicated in regulating many processes such as immune complex and pathogen clearance, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2 (By similarity). 
Sequence Annotation
 DOMAIN 19 122 Cystatin 1.
 DOMAIN 135 240 Cystatin 2.
 REGION 41 84 Interaction with ATP5A1 (By similarity).
 CARBOHYD 112 112 N-linked (GlcNAc...) (Potential).
 CARBOHYD 123 123 N-linked (GlcNAc...) (Potential).
 CARBOHYD 200 200 N-linked (GlcNAc...) (Potential).
 CARBOHYD 322 322 N-linked (GlcNAc...) (Potential).
 CARBOHYD 330 330 N-linked (GlcNAc...) (Potential).
 DISULFID 24 504 By similarity.
 DISULFID 78 89 By similarity.
 DISULFID 103 124 By similarity.
 DISULFID 201 414 By similarity.
 DISULFID 216 239 By similarity.  
Keyword
 Blood coagulation; Cleavage on pair of basic residues; Complete proteome; Copper; Disulfide bond; Fibrinolysis; Glycoprotein; Hemostasis; Heparin-binding; Metal-binding; Reference proteome; Repeat; Secreted; Signal; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 525 AA 
Protein Sequence
MKVLTTALLL VTLQCSHALS PTNCDASKPL AEKVLDLINK GRRSGYTFQL LRVSDAHLDR 60
VETATIYYLV LDVVESDCWV LSTKAQDECL PAMRTSEVVI GQCKVIATRY SNESQDLSVN 120
GYNCTMRSVS SAYINTKDSP VLVDSFEDSE PYRKLARKAL DKYKAENGDF ASFRVERAER 180
VIRMRGGERT SYFIEFSVRN CSTQHFPRHP PVFGLCRVVL TYSTEASDLE TPEYTDLICE 240
VFNTEDLKNR SDMKPHRGHE HPHCDKHLCK LSGPRDHHHT HKTHEIGCPP PPEGKDNSDR 300
PPLQEGALPQ MLPGHSGPSG TNRSHRPPHN HSCNEHPCHG QHPHGHHPHG QHPHGHHPHG 360
QHPHGHHPHG QHPHGHHPHG QHPHGHHPHG HHPHGDHPHG HHPHGHDFLD YGPCDPPSNS 420
QELKGQYHRG HGPPHGHSRK RGPGKGLFPF HQRQIGYVYR LPPLNVGEVL TPPEANFPIF 480
SLPNCNRPPQ PEIRPFPQTA SKSCPGKFEG KFPQVSNFFE HTPPK 525 
Gene Ontology
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:InterPro.
 GO:0020037; F:heme binding; ISS:UniProtKB.
 GO:0043395; F:heparan sulfate proteoglycan binding; ISS:UniProtKB.
 GO:0008201; F:heparin binding; ISS:UniProtKB.
 GO:0019865; F:immunoglobulin binding; ISS:UniProtKB.
 GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; ISS:UniProtKB.
 GO:0050832; P:defense response to fungus; ISS:UniProtKB.
 GO:0042730; P:fibrinolysis; IEA:UniProtKB-KW.
 GO:0016525; P:negative regulation of angiogenesis; ISS:UniProtKB.
 GO:0043537; P:negative regulation of blood vessel endothelial cell migration; ISS:UniProtKB.
 GO:0033629; P:negative regulation of cell adhesion mediated by integrin; ISS:UniProtKB.
 GO:0030308; P:negative regulation of cell growth; ISS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB.
 GO:2001027; P:negative regulation of endothelial cell chemotaxis; ISS:UniProtKB.
 GO:0051918; P:negative regulation of fibrinolysis; IEA:Compara.
 GO:0010593; P:negative regulation of lamellipodium assembly; ISS:UniProtKB.
 GO:1900747; P:negative regulation of vascular endothelial growth factor signaling pathway; ISS:UniProtKB.
 GO:0030168; P:platelet activation; ISS:UniProtKB.
 GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
 GO:2000504; P:positive regulation of blood vessel remodeling; ISS:UniProtKB.
 GO:0051894; P:positive regulation of focal adhesion assembly; ISS:UniProtKB.
 GO:0002839; P:positive regulation of immune response to tumor cell; ISS:UniProtKB.
 GO:0032956; P:regulation of actin cytoskeleton organization; ISS:UniProtKB.
 GO:0010468; P:regulation of gene expression; ISS:UniProtKB.
 GO:0050730; P:regulation of peptidyl-tyrosine phosphorylation; ISS:UniProtKB.
 GO:0010543; P:regulation of platelet activation; ISS:UniProtKB.
 GO:0043254; P:regulation of protein complex assembly; ISS:UniProtKB. 
Interpro
 IPR000010; Prot_inh_cystat. 
Pfam
  
SMART
 SM00043; CY 
PROSITE
 PS00287; CYSTATIN 
PRINTS