CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008393
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Methyl-CpG-binding protein 2 
Protein Synonyms/Alias
 MeCp-2 protein; MeCp2 
Gene Name
 MECP2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
130YLINPQGKAFRSKVEubiquitination[1]
321IEVKEVVKPLLVSTLacetylation[2]
449TAATAAEKYKHRGEGacetylation[3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 
Functional Description
 Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. 
Sequence Annotation
 DOMAIN 90 162 MBD.
 DNA_BIND 185 197 A.T hook 1.
 DNA_BIND 265 277 A.T hook 2.
 MOD_RES 80 80 Phosphoserine.
 MOD_RES 116 116 Phosphoserine.
 MOD_RES 216 216 Phosphoserine.
 MOD_RES 229 229 Phosphoserine (By similarity).
 MOD_RES 423 423 Phosphoserine (By similarity).
 MOD_RES 426 426 Phosphoserine.
 MOD_RES 449 449 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Chromosomal rearrangement; Complete proteome; Disease mutation; DNA-binding; Mental retardation; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 486 AA 
Protein Sequence
MVAGMLGLRE EKSEDQDLQG LKDKPLKFKK VKKDKKEEKE GKHEPVQPSA HHSAEPAEAG 60
KAETSEGSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY 120
DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPKSPK 180
APGTGRGRGR PKGSGTTRPK AATSEGVQVK RVLEKSPGKL LVKMPFQTSP GGKAEGGGAT 240
TSTQVMVIKR PGRKRKAEAD PQAIPKKRGR KPGSVVAAAA AEAKKKAVKE SSIRSVQETV 300
LPIKKRKTRE TVSIEVKEVV KPLLVSTLGE KSGKGLKTCK SPGRKSKESS PKGRSSSASS 360
PPKKEHHHHH HHSESPKAPV PLLPPLPPPP PEPESSEDPT SPPEPQDLSS SVCKEEKMPR 420
GGSLESDGCP KEPAKTQPAV ATAATAAEKY KHRGEGERKD IVSSSMPRPN REEPVDSRTP 480
VTERVS 486 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0000792; C:heterochromatin; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0010385; F:double-stranded methylated DNA binding; IMP:MGI.
 GO:0003729; F:mRNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0035197; F:siRNA binding; IEA:Compara.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0008344; P:adult locomotory behavior; IEA:Compara.
 GO:0001662; P:behavioral fear response; IEA:Compara.
 GO:0032048; P:cardiolipin metabolic process; IEA:Compara.
 GO:0050432; P:catecholamine secretion; IEA:Compara.
 GO:0021549; P:cerebellum development; IEA:Compara.
 GO:0006342; P:chromatin silencing; IEA:Compara.
 GO:0016358; P:dendrite development; IEA:Compara.
 GO:0009790; P:embryo development; IEA:Compara.
 GO:0008211; P:glucocorticoid metabolic process; IEA:Compara.
 GO:0006541; P:glutamine metabolic process; IEA:Compara.
 GO:0016573; P:histone acetylation; IEA:Compara.
 GO:0016571; P:histone methylation; IEA:Compara.
 GO:0006020; P:inositol metabolic process; IEA:Compara.
 GO:0007616; P:long-term memory; IEA:Compara.
 GO:0060291; P:long-term synaptic potentiation; IEA:Compara.
 GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IEA:Compara.
 GO:0035067; P:negative regulation of histone acetylation; IEA:Compara.
 GO:0031061; P:negative regulation of histone methylation; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
 GO:0001976; P:neurological system process involved in regulation of systemic arterial blood pressure; IEA:Compara.
 GO:0042551; P:neuron maturation; IEA:Compara.
 GO:0009405; P:pathogenesis; IEA:Compara.
 GO:0046470; P:phosphatidylcholine metabolic process; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0051965; P:positive regulation of synapse assembly; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0009791; P:post-embryonic development; IEA:Compara.
 GO:0019230; P:proprioception; IEA:Compara.
 GO:0008104; P:protein localization; IEA:Compara.
 GO:0060079; P:regulation of excitatory postsynaptic membrane potential; IEA:Compara.
 GO:0006349; P:regulation of gene expression by genetic imprinting; IEA:Compara.
 GO:0002087; P:regulation of respiratory gaseous exchange by neurological system process; IEA:Compara.
 GO:0007585; P:respiratory gaseous exchange; IEA:Compara.
 GO:0001666; P:response to hypoxia; IEA:Compara.
 GO:0019233; P:sensory perception of pain; IEA:Compara.
 GO:0035176; P:social behavior; IEA:Compara.
 GO:0001964; P:startle response; IEA:Compara.
 GO:0007416; P:synapse assembly; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0021591; P:ventricular system development; IEA:Compara.
 GO:0008542; P:visual learning; IEA:Compara. 
Interpro
 IPR017956; AT_hook_DNA-bd_motif.
 IPR016177; DNA-bd_integrase-typ.
 IPR017353; Me_CpG-bd_MeCP2.
 IPR001739; Methyl_CpG_DNA-bd. 
Pfam
 PF01429; MBD 
SMART
 SM00384; AT_hook
 SM00391; MBD 
PROSITE
 PS50982; MBD 
PRINTS