CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002311
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 3 subunit B 
Protein Synonyms/Alias
 eIF3b; Cell cycle regulation and translation initiation protein; Eukaryotic translation initiation factor 3 90 kDa subunit; eIF3 p90; Translation initiation factor eIF3 p90 subunit 
Gene Name
 PRT1 
Gene Synonyms/Alias
 CDC63; YOR361C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
197KSWLMDDKVRDQFVLubiquitination[1]
335VIKSPYLKWPLVRWSacetylation[2]
482ERDIPVEKVELKDSVacetylation[2]
587DMDYPGEKNINDNNDubiquitination[1]
638HKVEHGYKIFNIAGNacetylation[2]
726KIGQEMEKSMNFKIFubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. 
Sequence Annotation
 DOMAIN 77 162 RRM.
 REPEAT 228 266 WD 1.
 REPEAT 277 325 WD 2.
 REPEAT 373 416 WD 3.
 REPEAT 484 524 WD 4.
 REPEAT 544 589 WD 5.
 REPEAT 605 650 WD 6.
 REGION 1 136 Sufficient for interaction with HCR1 and
 REGION 28 261 Sufficient for interaction with PIC8.
 MOD_RES 61 61 Phosphoserine.
 MOD_RES 67 67 Phosphotyrosine.
 MOD_RES 669 669 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; Repeat; RNA-binding; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 763 AA 
Protein Sequence
MKNFLPRTLK NIYELYFNNI SVHSIVSRNT QLKRSKIIQM TTETFEDIKL EDIPVDDIDF 60
SDLEEQYKVT EEFNFDQYIV VNGAPVIPSA KVPVLKKALT SLFSKAGKVV NMEFPIDEAT 120
GKTKGFLFVE CGSMNDAKKI IKSFHGKRLD LKHRLFLYTM KDVERYNSDD FDTEFREPDM 180
PTFVPSSSLK SWLMDDKVRD QFVLQDDVKT SVFWNSMFNE EDSLVESREN WSTNYVRFSP 240
KGTYLFSYHQ QGVTAWGGPN FDRLRRFYHP DVRNSSVSPN EKYLVTFSTE PIIVEEDNEF 300
SPFTKKNEGH QLCIWDIASG LLMATFPVIK SPYLKWPLVR WSYNDKYCAR MVGDSLIVHD 360
ATKNFMPLEA KALKPSGIRD FSFAPEGVKL QPFRNGDEPS VLLAYWTPET NNSACTATIA 420
EVPRGRVLKT VNLVQVSNVT LHWQNQAEFL CFNVERHTKS GKTQFSNLQI CRLTERDIPV 480
EKVELKDSVF EFGWEPHGNR FVTISVHEVA DMNYAIPANT IRFYAPETKE KTDVIKRWSL 540
VKEIPKTFAN TVSWSPAGRF VVVGALVGPN MRRSDLQFYD MDYPGEKNIN DNNDVSASLK 600
DVAHPTYSAA TNITWDPSGR YVTAWSSSLK HKVEHGYKIF NIAGNLVKED IIAGFKNFAW 660
RPRPASILSN AERKKVRKNL REWSAQFEEQ DAMEADTAMR DLILHQRELL KQWTEYREKI 720
GQEMEKSMNF KIFDVQPEDA SDDFTTIEEI VEEVLEETKE KVE 763 
Gene Ontology
 GO:0010494; C:cytoplasmic stress granule; IDA:SGD.
 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:SGD.
 GO:0043614; C:multi-eIF complex; IDA:SGD.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IDA:SGD.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP. 
Interpro
 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR011400; EIF3B.
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR013979; TIF_beta_prop-like. 
Pfam
 PF08662; eIF2A
 PF00076; RRM_1 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS