CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009274
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Histidine--tRNA ligase 
Protein Synonyms/Alias
 Histidyl-tRNA synthetase; HisRS 
Gene Name
 hisS 
Gene Synonyms/Alias
 b2514; JW2498 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
30QRIEGTLKNVLGSYGacetylation[1]
53VEQTPLFKRAIGEVTacetylation[1, 2]
65EVTDVVEKEMYTFEDacetylation[1]
189VAFLEQHKEKLDEDCacetylation[1]
191FLEQHKEKLDEDCKRacetylation[1]
211PLRVLDSKNPEVQALacetylation[1]
370NHGGGNFKKQFARADacetylation[1]
371HGGGNFKKQFARADKacetylation[1]
378KQFARADKWGARVAVacetylation[1]
400ANGTAVVKDLRSGEQacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
  
Sequence Annotation
  
Keyword
 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 424 AA 
Protein Sequence
MAKNIQAIRG MNDYLPGETA IWQRIEGTLK NVLGSYGYSE IRLPIVEQTP LFKRAIGEVT 60
DVVEKEMYTF EDRNGDSLTL RPEGTAGCVR AGIEHGLLYN QEQRLWYIGP MFRHERPQKG 120
RYRQFHQLGC EVFGLQGPDI DAELIMLTAR WWRALGISEH VTLELNSIGS LEARANYRDA 180
LVAFLEQHKE KLDEDCKRRM YTNPLRVLDS KNPEVQALLN DAPALGDYLD EESREHFAGL 240
CKLLESAGIA YTVNQRLVRG LDYYNRTVFE WVTNSLGSQG TVCAGGRYDG LVEQLGGRAT 300
PAVGFAMGLE RLVLLVQAVN PEFKADPVVD IYLVASGADT QSAAMALAER LRDELPGVKL 360
MTNHGGGNFK KQFARADKWG ARVAVVLGES EVANGTAVVK DLRSGEQTAV AQDSVAAHLR 420
TLLG 424 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004821; F:histidine-tRNA ligase activity; IDA:EcoCyc.
 GO:0006427; P:histidyl-tRNA aminoacylation; IDA:EcoCyc. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR015807; His-tRNA-ligase.
 IPR004516; HisRS/HisZ. 
Pfam
 PF03129; HGTP_anticodon
 PF00587; tRNA-synt_2b 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS