CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020695
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pterin-4-alpha-carbinolamine dehydratase 2 
Protein Synonyms/Alias
 PHS 2; 4-alpha-hydroxy-tetrahydropterin dehydratase 2; DcoH-like protein DCoHm; Dimerization cofactor of hepatocyte nuclear factor 1 from muscle; HNF-1-alpha dimerization cofactor 
Gene Name
 Pcbd2 
Gene Synonyms/Alias
 Dcoh2; Dcohm 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
74IYKEFSFKNFNQAFGacetylation[1, 2, 3]
92RVALQAEKMNHHPEWacetylation[1, 3]
120HDCGGLTKRDVKLAQacetylation[2, 4]
120HDCGGLTKRDVKLAQsuccinylation[4]
124GLTKRDVKLAQFIEKacetylation[1, 2, 3, 4, 5, 6, 7, 8]
124GLTKRDVKLAQFIEKsuccinylation[4]
131KLAQFIEKAAASL**acetylation[2, 4, 5, 6, 7, 9]
131KLAQFIEKAAASL**succinylation[4]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [7] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [9] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199
Functional Description
 Involved in tetrahydrobiopterin biosynthesis. Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2 (By similarity). 
Sequence Annotation
  
Keyword
 3D-structure; Complete proteome; Lyase; Reference proteome; Tetrahydrobiopterin biosynthesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 136 AA 
Protein Sequence
MVAAAAVAVA AVGARSAGRW LAALRSPGAS RAAMSSDAQW LTAEERDQLI PGLKAAGWSE 60
LSERDAIYKE FSFKNFNQAF GFMSRVALQA EKMNHHPEWF NVYNKVQITL TSHDCGGLTK 120
RDVKLAQFIE KAAASL 136 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005634; C:nucleus; IPI:MGI.
 GO:0008124; F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity; IEA:EC.
 GO:0004505; F:phenylalanine 4-monooxygenase activity; IDA:MGI.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0051291; P:protein heterooligomerization; IPI:MGI.
 GO:0051289; P:protein homotetramerization; IPI:MGI.
 GO:0006729; P:tetrahydrobiopterin biosynthetic process; IEA:UniProtKB-KW. 
Interpro
 IPR001533; Trans/pterin_deHydtase. 
Pfam
 PF01329; Pterin_4a 
SMART
  
PROSITE
  
PRINTS