| Tag | Content |
|---|
| CPLM ID | CPLM-021156 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Probable alanine--tRNA ligase, chloroplastic |
Protein Synonyms/Alias | Alanyl-tRNA synthetase; AlaRS; Protein EMBRYO DEFECTIVE 1030; Protein EMBRYO DEFECTIVE 263; Protein EMBRYO DEFECTIVE 86 |
Gene Name | EMB86 |
Gene Synonyms/Alias | EMB1030; EMB263; At5g22800; MRN17.3 |
Created Date | July 27, 2013 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
NCBI Taxa ID | 3702 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 773 | TAEIRAFKIISEQGI | ubiquitination | [1] |
|
Reference | [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants. Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K. Mol Cell Proteomics. 2007 Apr;6(4):601-10. [ PMID: 17272265] |
Functional Description | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain (By similarity). |
Sequence Annotation | METAL 655 655 Zinc (Potential).
METAL 659 659 Zinc (Potential).
METAL 758 758 Zinc (Potential).
METAL 762 762 Zinc (Potential).
CROSSLNK 773 773 Glycyl lysine isopeptide (Lys-Gly) |
Keyword | Aminoacyl-tRNA synthetase; ATP-binding; Chloroplast; Complete proteome; Isopeptide bond; Ligase; Metal-binding; Mitochondrion; Nucleotide-binding; Plastid; Protein biosynthesis; Reference proteome; RNA-binding; Transit peptide; tRNA-binding; Ubl conjugation; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 978 AA |
Protein Sequence | MNFSRVNLFD FPLRPILLSH PSSIFVSTRF VTRTSAGVSP SILLPRSTQS PQIIAKSSSV 60
SVQPVSEDAK EDYQSKDVSG DSIRRRFLEF FASRGHKVLP SSSLVPEDPT VLLTIAGMLQ 120
FKPIFLGKVP REVPCATTAQ RCIRTNDLEN VGKTARHHTF FEMLGNFSFG DYFKKEAIKW 180
AWELSTIEFG LPANRVWVSI YEDDDEAFEI WKNEVGVSVE RIKRMGEADN FWTSGPTGPC 240
GPCSELYYDF YPERGYDEDV DLGDDTRFIE FYNLVFMQYN KTEDGLLEPL KQKNIDTGLG 300
LERIAQILQK VPNNYETDLI YPIIAKISEL ANISYDSAND KAKTSLKVIA DHMRAVVYLI 360
SDGVSPSNIG RGYVVRRLIR RAVRKGKSLG INGDMNGNLK GAFLPAVAEK VIELSTYIDS 420
DVKLKASRII EEIRQEELHF KKTLERGEKL LDQKLNDALS IADKTKDTPY LDGKDAFLLY 480
DTFGFPVEIT AEVAEERGVS IDMNGFEVEM ENQRRQSQAA HNVVKLTVED DADMTKNIAD 540
TEFLGYDSLS ARAVVKSLLV NGKPVIRVSE GSEVEVLLDR TPFYAESGGQ IADHGFLYVS 600
SDGNQEKAVV EVSDVQKSLK IFVHKGTVKS GALEVGKEVE AAVDADLRQR AKVHHTATHL 660
LQSALKKVVG QETSQAGSLV AFDRLRFDFN FNRSLHDNEL EEIECLINRW IGDATRLETK 720
VLPLADAKRA GAIAMFGEKY DENEVRVVEV PGVSMELCGG THVGNTAEIR AFKIISEQGI 780
ASGIRRIEAV AGEAFIEYIN SRDSQMTRLC STLKVKAEDV TNRVENLLEE LRAARKEASD 840
LRSKAAVYKA SVISNKAFTV GTSQTIRVLV ESMDDTDADS LKSAAEHLIS TLEDPVAVVL 900
GSSPEKDKVS LVAAFSPGVV SLGVQAGKFI GPIAKLCGGG GGGKPNFAQA GGRKPENLPS 960
ALEKAREDLV ATLSEKLG 978 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |