CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008752
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pituitary tumor-transforming gene 1 protein-interacting protein 
Protein Synonyms/Alias
 Pituitary tumor-transforming gene protein-binding factor; PBF; PTTG-binding factor 
Gene Name
 PTTG1IP 
Gene Synonyms/Alias
 C21orf1; C21orf3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
164RHDEIRKKYGLFKEEubiquitination[1, 2, 3, 4]
169RKKYGLFKEENPYARubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 May facilitate PTTG1 nuclear translocation. 
Sequence Annotation
 DOMAIN 39 92 PSI.
 MOD_RES 174 174 Phosphotyrosine (By similarity).
 CARBOHYD 45 45 N-linked (GlcNAc...) (Potential).
 CARBOHYD 54 54 N-linked (GlcNAc...) (Potential).  
Keyword
 Coiled coil; Complete proteome; Cytoplasm; Glycoprotein; Membrane; Nucleus; Phosphoprotein; Reference proteome; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 180 AA 
Protein Sequence
MAPGVARGPT PYWRLRLGGA ALLLLLIPVA AAQEPPGAAC SQNTNKTCEE CLKNVSCLWC 60
NTNKACLDYP VTSVLPPASL CKLSSARWGV CWVNFEALII TMSVVGGTLL LGIAICCCCC 120
CRRKRSRKPD RSEEKAMRER EERRIRQEER RAEMKTRHDE IRKKYGLFKE ENPYARFENN 180 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0016020; C:membrane; NAS:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0006606; P:protein import into nucleus; IDA:UniProtKB. 
Interpro
 IPR003659; Plexin-like. 
Pfam
  
SMART
 SM00423; PSI 
PROSITE
  
PRINTS