UniProt Accession

 SIR2_RAT; Q5RJQ4 

Genbank Protein ID

 BC086545 

Genbank Nucleotide ID

 AAH86545.1 

Protein Name

 NAD-dependent protein deacetylase sirtuin-2 

Protein Synonyms/Alias

 Regulatory protein SIR2 homolog 2; SIR2-like protein 2 

Gene Name

 Sirt2 

Gene Synonyms/Alias

 Sir2l2 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
18	TLGLGSQKERLLDEL	acetylation	[1]
18	TLGLGSQKERLLDEL	ubiquitination	[2]
89	FEISYFKKHPEPFFA	ubiquitination	[2]
121	FIRLLKEKGLLLRCY	acetylation	[1]
173	EYTMSWMKEKIFSEA	acetylation	[1]
175	TMSWMKEKIFSEATP	acetylation	[1]
183	IFSEATPKCEKCQNV	acetylation	[1]
250	TPRLLINKEKTGQTD	acetylation	[1]
250	TPRLLINKEKTGQTD	ubiquitination	[2]
301	LADLLGWKELEDLVR	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA (By similarity). 

Sequence Annotation

 DOMAIN 28 303 Deacetylase sirtuin-type.
 NP_BIND 47 67 NAD (By similarity).
 NP_BIND 130 133 NAD (By similarity).
 NP_BIND 224 226 NAD (By similarity).
 NP_BIND 249 251 NAD (By similarity).
 ACT_SITE 150 150 Proton acceptor (By similarity).
 METAL 158 158 Zinc (By similarity).
 METAL 163 163 Zinc (By similarity).
 METAL 184 184 Zinc (By similarity).
 METAL 187 187 Zinc (By similarity).
 BINDING 287 287 NAD; via amide nitrogen (By similarity).  

Keyword

 Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Hydrolase; Metal-binding; Microtubule; Mitosis; NAD; Phosphoprotein; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 350 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0070403; F:NAD+ binding; IEA:InterPro.
 GO:0017136; F:NAD-dependent histone deacetylase activity; IEA:Compara.
 GO:0042903; F:tubulin deacetylase activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:Compara.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0045843; P:negative regulation of striated muscle tissue development; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB. 

Interpro

 IPR017328; NAD-dep_deAcase_SIR2_class_I.
 IPR003000; Sirtuin.
 IPR026590; Ssirtuin_cat_dom. 

Pfam

 PF02146; SIR2 

SMART

  

PROSITE

 PS50305; SIRTUIN 

PRINTS