UniProt Accession

 PSA5_YEAST; P32379; D6VV33 

Genbank Protein ID

 X64918; X99228; Z73038; AY558511; BK006941 

Genbank Nucleotide ID

 CAA46111.1; CAA67615.1; CAA97282.1; AAS56837.1; DAA08344.1 

Protein Name

 Proteasome subunit alpha type-5 

Protein Synonyms/Alias

 Macropain subunit PUP2; Multicatalytic endopeptidase complex subunit PUP2; Proteasome component PUP2; Proteinase YSCE subunit PUP2 

Gene Name

 PUP2 

Gene Synonyms/Alias

 DOA5; YGR253C; G9155 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
170	TFYRYNAKAIGSGSE	ubiquitination	[1]
202	EAELLVLKILKQVME	ubiquitination	[1]
205	LLVLKILKQVMEEKL	acetylation	[2]
205	LLVLKILKQVMEEKL	ubiquitination	[1]
211	LKQVMEEKLDENNAQ	ubiquitination	[1]
224	AQLSCITKQDGFKIY	ubiquitination	[1]
235	FKIYDNEKTAELIKE	ubiquitination	[1]
246	LIKELKEKEAAESPE	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. 

Sequence Annotation

 MOD_RES 55 55 Phosphothreonine.
 MOD_RES 56 56 Phosphoserine.
 MOD_RES 251 251 Phosphoserine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Nucleus; Phosphoprotein; Protease; Proteasome; Reference proteome; Threonine protease. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 260 AA 

Protein Sequence

Gene Ontology

 GO:0042175; C:nuclear outer membrane-endoplasmic reticulum membrane network; IC:SGD.
 GO:0005634; C:nucleus; IC:SGD.
 GO:0019773; C:proteasome core complex, alpha-subunit complex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0010499; P:proteasomal ubiquitin-independent protein catabolic process; IDA:SGD. 

Interpro

 IPR000426; Proteasome_asu_N.
 IPR023332; Proteasome_suA-type.
 IPR001353; Proteasome_sua/b. 

Pfam

 PF00227; Proteasome
 PF10584; Proteasome_A_N 

SMART

 SM00948; Proteasome_A_N 

PROSITE

 PS00388; PROTEASOME_A_1
 PS51475; PROTEASOME_A_2 

PRINTS