CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012161
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sorting nexin-1 
Protein Synonyms/Alias
  
Gene Name
 SNX1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
196DFLGLYEKLSEKHSQubiquitination[1, 2]
200LYEKLSEKHSQNGFIubiquitination[2]
237SSAEFLEKRRAALERacetylation[3]
267DVREFLEKEELPRAVubiquitination[4]
286LSGAGLLKMFNKATDubiquitination[1, 2, 4]
290GLLKMFNKATDAVSKubiquitination[2]
297KATDAVSKMTIKMNEubiquitination[2]
325CEEQRLRKLHAVVETubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3- phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). 
Sequence Annotation
 DOMAIN 143 272 PX.
 DOMAIN 302 522 BAR.
 BINDING 186 186 Phosphatidylinositol 3-phosphate (By
 BINDING 188 188 Phosphatidylinositol 3-phosphate; via
 BINDING 214 214 Phosphatidylinositol 3-phosphate (By
 BINDING 238 238 Phosphatidylinositol 3-phosphate (By
 MOD_RES 32 32 Phosphoserine.
 MOD_RES 39 39 Phosphoserine.
 MOD_RES 188 188 Phosphoserine.
 MOD_RES 237 237 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Endosome; Golgi apparatus; Lipid-binding; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 522 AA 
Protein Sequence
MASGGGGCSA SERLPPPFPG LEPESEGAAG GSEPEAGDSD TEGEDIFTGA AVVSKHQSPK 60
ITTSLLPINN GSKENGIHEE QDQEPQDLFA DATVELSLDS TQNNQKKVLA KTLISLPPQE 120
ATNSSKPQPT YEELEEEEQE DQFDLTVGIT DPEKIGDGMN AYVAYKVTTQ TSLPLFRSKQ 180
FAVKRRFSDF LGLYEKLSEK HSQNGFIVPP PPEKSLIGMT KVKVGKEDSS SAEFLEKRRA 240
ALERYLQRIV NHPTMLQDPD VREFLEKEEL PRAVGTQTLS GAGLLKMFNK ATDAVSKMTI 300
KMNESDIWFE EKLQEVECEE QRLRKLHAVV ETLVNHRKEL ALNTAQFAKS LAMLGSSEDN 360
TALSRALSQL AEVEEKIEQL HQEQANNDFF LLAELLSDYI RLLAIVRAAF DQRMKTWQRW 420
QDAQATLQKK REAEARLLWA NKPDKLQQAK DEILEWESRV TQYERDFERI STVVRKEVIR 480
FEKEKSKDFK NHVIKYLETL LYSQQQLAKY WEAFLPEAKA IS 522 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0031901; C:early endosome membrane; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0035091; F:phosphatidylinositol binding; IDA:UniProtKB.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0034498; P:early endosome to Golgi transport; IMP:UniProtKB.
 GO:0006897; P:endocytosis; TAS:ProtInc.
 GO:0006886; P:intracellular protein transport; IMP:UniProtKB.
 GO:0045732; P:positive regulation of protein catabolic process; IEA:Compara. 
Interpro
 IPR001683; Phox.
 IPR005329; Sorting_nexin_N.
 IPR015404; Vps5_C. 
Pfam
 PF00787; PX
 PF03700; Sorting_nexin
 PF09325; Vps5 
SMART
 SM00312; PX 
PROSITE
 PS51021; BAR
 PS50195; PX 
PRINTS