CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008194
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar protein sorting-associated protein 41 homolog 
Protein Synonyms/Alias
 S53 
Gene Name
 VPS41 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
200KIFDIISKQRITNVPubiquitination[1]
358PRDVVVAKERDQDDHubiquitination[1]
553HNLFSSIKDKIVLLMubiquitination[1, 2]
642STHCPLEKALEICQQubiquitination[1]
671GNSRSALKMIMEELHubiquitination[1, 2]
731ILLIHRIKEGMEIPNubiquitination[1]
761ILLREGCKKILVADSacetylation[3]
762LLREGCKKILVADSLubiquitination[4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Required for vacuolar assembly and vacuolar traffic. 
Sequence Annotation
 REPEAT 568 712 CHCR.
 ZN_FING 791 839 RING-type; atypical.  
Keyword
 Alternative splicing; Complete proteome; Metal-binding; Polymorphism; Protein transport; Reference proteome; Transport; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 854 AA 
Protein Sequence
MAEAEEQETG SLEESTDESE EEESEEEPKL KYERLSNGVT EILQKDAASC MTVHDKFLAL 60
GTHYGKVYLL DVQGNITQKF DVSPVKINQI SLDESGEHMG VCSEDGKVQV FGLYSGEEFH 120
ETFDCPIKII AVHPHFVRSS CKQFVTGGKK LLLFERSWMN RWKSAVLHEG EGNIRSVKWR 180
GHLIAWANNM GVKIFDIISK QRITNVPRDD ISLRPDMYPC SLCWKDNVTL IIGWGTSVKV 240
CSVKERHASE MRDLPSRYVE IVSQFETEFY ISGLAPLCDQ LVVLSYVKEI SEKTEREYCA 300
RPRLDIIQPL SETCEEISSD ALTVRGFQEN ECRDYHLEYS EGESLFYIVS PRDVVVAKER 360
DQDDHIDWLL EKKKYEEALM AAEISQKNIK RHKILDIGLA YINHLVERGD YDIAARKCQK 420
ILGKNAALWE YEVYKFKEIG QLKAISPYLP RGDPVLKPLI YEMILHEFLE SDYEGFATLI 480
REWPGDLYNN SVIVQAVRDH LKKDSQNKTL LKTLAELYTY DKNYGNALEI YLTLRHKDVF 540
QLIHKHNLFS SIKDKIVLLM DFDSEKAVDM LLDNEDKISI KKVVEELEDR PELQHVYLHK 600
LFKRDHHKGQ RYHEKQISLY AEYDRPNLLP FLRDSTHCPL EKALEICQQR NFVEETVYLL 660
SRMGNSRSAL KMIMEELHDV DKAIEFAKEQ DDGELWEDLI LYSIDKPPFI TGLLNNIGTH 720
VDPILLIHRI KEGMEIPNLR DSLVKILQDY NLQILLREGC KKILVADSLS LLKKMHRTQM 780
KGVLVDEENI CESCLSPILP SDAAKPFSVV VFHCRHMFHK ECLPMPSMNS AAQFCNICSA 840
KNRGPGSAIL EMKK 854 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005769; C:early endosome; IEA:Compara.
 GO:0005798; C:Golgi-associated vesicle; IMP:UniProtKB.
 GO:0030897; C:HOPS complex; IDA:UniProtKB.
 GO:0005770; C:late endosome; IEA:Compara.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0005874; C:microtubule; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0048193; P:Golgi vesicle transport; IMP:UniProtKB.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR000547; Clathrin_H-chain/VPS_repeat.
 IPR011990; TPR-like_helical.
 IPR016902; VPS41.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR017986; WD40_repeat_dom.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF00637; Clathrin 
SMART
 SM00299; CLH
 SM00184; RING 
PROSITE
 PS50236; CHCR
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS