CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003482
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NAD(P)H-flavin reductase 
Protein Synonyms/Alias
 Aquacobalamin reductase; FMN reductase; Ferrisiderophore reductase C; NAD(P)H:flavin oxidoreductase; Riboflavin reductase [NAD(P)H] 
Gene Name
 fre 
Gene Synonyms/Alias
 fadI; flrD; fsrC; ubiB; b3844; JW3820 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
46VVMDERDKRPFSMASacetylation[1]
208AGRFEMAKIARDLFCacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618
Functional Description
 Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Seems to reduce the complexed Fe(3+) iron of siderophores to Fe(2+), thus releasing it from the chelator. 
Sequence Annotation
 DOMAIN 2 99 FAD-binding FR-type.
 NP_BIND 111 115 Pyridine (By similarity).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; FAD; Flavoprotein; FMN; Ion transport; Iron; Iron transport; NAD; NADP; Oxidoreductase; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 233 AA 
Protein Sequence
MTTLSCKVTS VEAITDTVYR VRIVPDAAFS FRAGQYLMVV MDERDKRPFS MASTPDEKGF 60
IELHIGASEI NLYAKAVMDR ILKDHQIVVD IPHGEAWLRD DEERPMILIA GGTGFSYARS 120
ILLTALARNP NRDITIYWGG REEQHLYDLC ELEALSLKHP GLQVVPVVEQ PEAGWRGRTG 180
TVLTAVLQDH GTLAEHDIYI AGRFEMAKIA RDLFCSERNA REDRLFGDAF AFI 233 
Gene Ontology
 GO:0047138; F:aquacobalamin reductase activity; IEA:EC.
 GO:0052875; F:riboflavin reductase (NADH) activity; IEA:EC.
 GO:0042602; F:riboflavin reductase (NADPH) activity; IEA:EC.
 GO:0006811; P:ion transport; IEA:UniProtKB-KW.
 GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW. 
Interpro
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR001221; Phe_hydroxylase.
 IPR017938; Riboflavin_synthase-like_b-brl. 
Pfam
 PF00970; FAD_binding_6
 PF00175; NAD_binding_1 
SMART
  
PROSITE
 PS51384; FAD_FR 
PRINTS
 PR00410; PHEHYDRXLASE.