CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012110
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Unconventional myosin-IXb 
Protein Synonyms/Alias
 Unconventional myosin-9b 
Gene Name
 MYO9B 
Gene Synonyms/Alias
 MYR5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MSVKEAGSSGRubiquitination[1]
262CLTALSQKGYASGVEubiquitination[1]
844KLLEALGKAEPFFIRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. May be involved in the remodeling of the actin cytoskeleton. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activating protein on Rho. 
Sequence Annotation
 DOMAIN 1 939 Myosin head-like.
 DOMAIN 15 114 Ras-associating.
 DOMAIN 957 977 IQ 1.
 DOMAIN 979 1000 IQ 2.
 DOMAIN 1001 1023 IQ 3.
 DOMAIN 1024 1053 IQ 4.
 DOMAIN 1703 1888 Rho-GAP.
 NP_BIND 239 246 ATP (Potential).
 ZN_FING 1632 1681 Phorbol-ester/DAG-type.
 REGION 844 855 Actin-binding.
 REGION 940 1044 Neck or regulatory domain.
 REGION 1045 2157 Tail.
 MOD_RES 716 716 Phosphoserine.
 MOD_RES 1242 1242 Phosphoserine (By similarity).
 MOD_RES 1267 1267 Phosphoserine.
 MOD_RES 1271 1271 Phosphothreonine.
 MOD_RES 1290 1290 Phosphoserine.
 MOD_RES 1323 1323 Phosphoserine.
 MOD_RES 1346 1346 Phosphothreonine.
 MOD_RES 1348 1348 Phosphothreonine (By similarity).
 MOD_RES 1354 1354 Phosphoserine.
 MOD_RES 1405 1405 Phosphoserine.
 MOD_RES 1992 1992 Phosphoserine.  
Keyword
 Actin-binding; Alternative splicing; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; GTPase activation; Metal-binding; Motor protein; Myosin; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2157 AA 
Protein Sequence
MSVKEAGSSG RREQAAYHLH IYPQLSTTES QASCRVTATK DSTTSDVIKD AIASLRLDGT 60
KCYVLVEVKE SGGEEWVLDA NDSPVHRVLL WPRRAQDEHP QEDGYYFLLQ ERNADGTIKY 120
VHMQLVAQAT ATRRLVERGL LPRQQADFDD LCNLPELTEG NLLKNLKHRF LQQKIYTYAG 180
SILVAINPFK FLPIYNPKYV KMYENQQLGK LEPHVFALAD VAYYTMLRKR VNQCIVISGE 240
SGSGKTQSTN FLIHCLTALS QKGYASGVER TILGAGPVLE AFGNAKTAHN NNSSRFGKFI 300
QVSYLESGIV RGAVVEKYLL EKSRLVSQEK DERNYHVFYY LLLGVSEEER QEFQLKQPED 360
YFYLNQHNLK IEDGEDLKHD FERLKQAMEM VGFLPATKKQ IFAVLSAILY LGNVTYKKRA 420
TGREEGLEVG PPEVLDTLSQ LLKVKREILV EVLTKRKTVT VNDKLILPYS LSEAITARDS 480
MAKSLYSALF DWIVLRINHA LLNKKDVEEA VSCLSIGVLD IFGFEDFERN SFEQFCINYA 540
NEQLQYYFNQ HIFKLEQEEY QGEGITWHNI GYTDNVGCIH LISKKPTGLF YLLDEESNFP 600
HATSQTLLAK FKQQHEDNKY FLGTPVMEPA FIIQHFAGKV KYQIKDFREK NMDYMRPDIV 660
ALLRGSDSSY VRELIGMDPV AVFRWAVLRA AIRAMAVLRE AGRLRAERAE KAAGMSSPGA 720
QSHPEELPRG ASTPSEKLYR DLHNQMIKSI KGLPWQGEDP RSLLQSLSRL QKPRAFILKS 780
KGIKQKQIIP KNLLDSKSLK LIISMTLHDR TTKSLLHLHK KKKPPSISAQ FQTSLNKLLE 840
ALGKAEPFFI RCIRSNAEKK ELCFDDELVL QQLRYTGMLE TVRIRRSGYS AKYTFQDFTE 900
QFQVLLPKDA QPCREVISTL LEKMKIDKRN YQIGKTKVFL KETERQALQE TLHREVVRKI 960
LLLQSWFRMV LERRHFLQMK RAAVTIQACW RSYRVRRALE RTQAAVYLQA SWRGYWQRKL 1020
YRHQKQSIIR LQSLCRGHLQ RKSFSQMISE KQKAEEKERE ALEAARAGAE EGGQGQAAGG 1080
QQVAEQGPEP AEDGGHLASE PEVQPSDRSP LEHSSPEKEA PSPEKTLPPQ KTVAAESHEK 1140
VPSSREKRES RRQRGLEHVK FQNKHIQSCK EESALREPSR RVTQEQGVSL LEDKKESRED 1200
ETLLVVETEA ENTSQKQPTE QPQAMAVGKV SEETEKTLPS GSPRPGQLER PTSLALDSRV 1260
SPPAPGSAPE TPEDKSKPCG SPRVQEKPDS PGGSTQIQRY LDAERLASAV ELWRGKKLVA 1320
AASPSAMLSQ SLDLSDRHRA TGAALTPTEE RRTSFSTSDV SKLLPSLAKA QPAAETTDGE 1380
RSAKKPAVQK KKPGDASSLP DAGLSPGSQV DSKSTFKRLF LHKTKDKKYS LEGAEELENA 1440
VSGHVVLEAT TMKKGLEAPS GQQHRHAAGE KRTKEPGGKG KKNRNVKIGK ITVSEKWRES 1500
VFRQITNANE LKYLDEFLLN KINDLRSQKT PIESLFIEAT EKFRSNIKTM YSVPNGKIHV 1560
GYKDLMENYQ IVVSNLATER GQKDTNLVLN LFQSLLDEFT RGYTKNDFEP VKQSKAQKKK 1620
RKQERAVQEH NGHVFASYQV SIPQSCEQCL SYIWLMDKAL LCSVCKMTCH KKCVHKIQSH 1680
CSYTYGRKGE PGVEPGHFGV CVDSLTSDKA SVPIVLEKLL EHVEMHGLYT EGLYRKSGAA 1740
NRTRELRQAL QTDPAAVKLE NFPIHAITGV LKQWLRELPE PLMTFAQYGD FLRAVELPEK 1800
QEQLAAIYAV LEHLPEANHN SLERLIFHLV KVALLEDVNR MSPGALAIIF APCLLRCPDN 1860
SDPLTSMKDV LKITTCVEML IKEQMRKYKV KMEEISQLEA AESIAFRRLS LLRQNAPWPL 1920
KLGFSSPYEG VLNKSPKTRD IQEEELEVLL EEEAAGGDED REKEILIERI QSIKEEKEDI 1980
TYRLPELDPR GSDEENLDSE TSASTESLLE ERAGRGASEG PPAPALPCPG APTPSPLPTV 2040
AAPPRRRPSS FVTVRVKTPR RTPIMPTANI KLPPGLPSHL PRWAPGAREA AAPVRRREPP 2100
ARRPDQIHSV YITPGADLPV QGALEPLEED GQPPGAKRRY SDPPTYCLPP ASGQTNG 2157 
Gene Ontology
 GO:0005938; C:cell cortex; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031941; C:filamentous actin; IDA:UniProtKB.
 GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0003779; F:actin binding; IDA:UniProtKB.
 GO:0043531; F:ADP binding; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0016887; F:ATPase activity; IDA:UniProtKB.
 GO:0005516; F:calmodulin binding; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000146; F:microfilament motor activity; IDA:UniProtKB.
 GO:0005100; F:Rho GTPase activator activity; IDA:UniProtKB.
 GO:0030048; P:actin filament-based movement; TAS:UniProtKB.
 GO:0007266; P:Rho protein signal transduction; IC:UniProtKB. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR001609; Myosin_head_motor_dom.
 IPR027417; P-loop_NTPase.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000159; Ras-assoc.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00612; IQ
 PF00063; Myosin_head
 PF00788; RA
 PF00620; RhoGAP 
SMART
 SM00109; C1
 SM00015; IQ
 SM00242; MYSc
 SM00314; RA
 SM00324; RhoGAP 
PROSITE
 PS50096; IQ
 PS50200; RA
 PS50238; RHOGAP
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS
 PR00193; MYOSINHEAVY.