UniProt Accession

 ABP1_YEAST; P15891; D6VR88 

Genbank Protein ID

 X51780; X59720; BK006937 

Genbank Nucleotide ID

 CAA36075.1; CAA42253.1; DAA07557.1 

Protein Name

 Actin-binding protein 

Protein Synonyms/Alias

  

Gene Name

 ABP1 

Gene Synonyms/Alias

 YCR088W; YCR88W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
94	CPDSAPLKTRASFAA	ubiquitination	[1]
150	YSIQTSSKQQGKAST	ubiquitination	[1]
189	PSPAPAAKISSRVND	ubiquitination	[1]
209	DWNEPELKERDFDQA	ubiquitination	[1]
333	NSGNAETKAEAPKPE	ubiquitination	[1]
338	ETKAEAPKPEVPEDE	ubiquitination	[1, 2]
353	PEGEPDVKDLKSKFE	ubiquitination	[1]
358	DVKDLKSKFEGLAAS	acetylation	[3]
395	ISPKPFSKPQEPVKA	acetylation	[3]
464	ASGAPVQKEEPEQEE	ubiquitination	[1, 2]
486	RNSIPAPKQEEAPEQ	ubiquitination	[1]
580	ELEKDGSKGLFPSNY	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Regulates ARP2/3 complex-mediated actin assembly. Recruits ARP2/3 complex to sides of preexisting actin filaments, which may promote nucleation or stabilization of filament branches. Binds to actin filaments, but not actin monomers. Actin binding is required for ARP2/3 complex activation. May also have a role in linking the actin cytoskeleton to endocytosis. recruits components of the endocytotic machinery to cortical actin patches, known sites of endocytosis. 

Sequence Annotation

 DOMAIN 7 136 ADF-H.
 REPEAT 200 209 1.
 REPEAT 436 445 2.
 DOMAIN 532 592 SH3.
 REPEAT 566 575 3.
 REGION 200 575 3 X 10 AA approximate repeats (acidic).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 165 165 Phosphothreonine.
 MOD_RES 167 167 Phosphoserine.
 MOD_RES 169 169 Phosphoserine.
 MOD_RES 181 181 Phosphothreonine.
 MOD_RES 183 183 Phosphoserine.
 MOD_RES 313 313 Phosphoserine.
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 389 389 Phosphoserine.
 MOD_RES 458 458 Phosphoserine.
 MOD_RES 481 481 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein; Reference proteome; Repeat; SH3 domain. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 592 AA 

Protein Sequence

Gene Ontology

 GO:0030479; C:actin cortical patch; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0051015; F:actin filament binding; IDA:SGD.
 GO:0000147; P:actin cortical patch assembly; IMP:SGD.
 GO:0008104; P:protein localization; IMP:SGD. 

Interpro

 IPR002108; Actin-bd_cofilin/tropomyosin.
 IPR000108; p67phox.
 IPR001452; SH3_domain. 

Pfam

 PF00241; Cofilin_ADF
 PF00018; SH3_1 

SMART

 SM00102; ADF
 SM00326; SH3 

PROSITE

 PS51263; ADF_H
 PS50002; SH3 

PRINTS

 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.