UniProt Accession

 SYFB_HUMAN; Q9NSD9; O95708; Q4ZFX1; Q57ZJ5; Q9NZZ6 

Genbank Protein ID

 D84430; AF042346; AF161521; AC097461; AC104772; BC017783 

Genbank Nucleotide ID

 BAA95608.1; AAD02220.1; AAF29136.1; AAX88958.1; AAX81986.1; AAH17783.1 

Protein Name

 Phenylalanine--tRNA ligase beta subunit 

Protein Synonyms/Alias

 Phenylalanyl-tRNA synthetase beta subunit; PheRS 

Gene Name

 FARSB 

Gene Synonyms/Alias

 FARSLB; FRSB; HSPC173 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
47	SEKEIISKEQGNVKA	ubiquitination	[1]
53	SKEQGNVKAAGASDV	ubiquitination	[1, 2, 3]
64	ASDVVLYKIDVPANR	ubiquitination	[1, 2, 3, 4]
88	VRGLQVFKERIKAPV	ubiquitination	[2]
97	RIKAPVYKRVMPDGK	ubiquitination	[2, 3]
107	MPDGKIQKLIITEET	ubiquitination	[2]
145	SFIELQEKLHQNICR	ubiquitination	[2]
329	ETPENLAKLLTRMYL	acetylation	[5]
329	ETPENLAKLLTRMYL	ubiquitination	[2, 4]
395	ANQFPLNKLTELLRH	ubiquitination	[1, 2]
443	AVHISNPKTAEFQVA	ubiquitination	[2]
460	TLLPGLLKTIAANRK	ubiquitination	[1, 2]
483	EISDIVIKDSNTDVG	ubiquitination	[1]
492	SNTDVGAKNYRHLCA	ubiquitination	[1, 2]
530	DVPPGEDKGGYVIKA	ubiquitination	[1]
536	DKGGYVIKASEGPAF	ubiquitination	[1]
560	ARGQSVGKLGVLHPD	ubiquitination	[1, 2, 4, 6]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

  

Sequence Annotation

 DOMAIN 302 379 B5.  

Keyword

 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 589 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004826; F:phenylalanine-tRNA ligase activity; TAS:ProtInc.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0006432; P:phenylalanyl-tRNA aminoacylation; IEA:InterPro.
 GO:0006418; P:tRNA aminoacylation for protein translation; TAS:Reactome. 

Interpro

 IPR005146; B3/B4_tRNA-bd.
 IPR009061; DNA-bd_dom_put.
 IPR004531; Phe-tRNA-synth_IIc_bsu_arc.
 IPR020825; Phe-tRNA_synthase_B3/B4.
 IPR005147; tRNA_synthase_B5-dom. 

Pfam

 PF03483; B3_4
 PF03484; B5 

SMART

 SM00873; B3_4
 SM00874; B5 

PROSITE

 PS51483; B5 

PRINTS