CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013452
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleoporin GLE1 
Protein Synonyms/Alias
 hGLE1; GLE1-like protein 
Gene Name
 GLE1 
Gene Synonyms/Alias
 GLE1L 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
12GRCWETLKALRSSDKubiquitination[1]
107PATPNGTKGKDESQHubiquitination[1, 2]
109TPNGTKGKDESQHTEubiquitination[1, 2, 3]
127LQSSRGIKVEGCVRMubiquitination[1]
142YELVHRMKGTEGLRLubiquitination[1]
157WQEEQERKVQALSEMubiquitination[1]
170EMASEQLKRFDEWKEubiquitination[1, 2, 3]
176LKRFDEWKELKQHKEubiquitination[1, 2, 3]
179FDEWKELKQHKEFQDubiquitination[1]
182WKELKQHKEFQDLREubiquitination[1]
204EALGHQEKLKAEHRHubiquitination[1, 3]
214AEHRHRAKILNLKLRubiquitination[1, 3]
238AEQERLRKEEGQIRLubiquitination[1]
269LDASEQHKALLKVDLubiquitination[1]
273EQHKALLKVDLAAFQubiquitination[1]
361QQGPEAHKEPPAPSQubiquitination[1, 2]
373PSQGPGGKQNEDLQVubiquitination[1, 2]
419DSQAKKIKMDLQKAAubiquitination[1]
424KIKMDLQKAATIPVSubiquitination[1, 2]
442TIAGSKLKEIFDKIHubiquitination[1]
455IHSLLSGKPVQSGGRubiquitination[1, 2]
479GLDFVQYKLAEKFVKubiquitination[1, 2]
483VQYKLAEKFVKQGEEubiquitination[1]
486KLAEKFVKQGEEEVAubiquitination[1]
526LILAHLHKKCPYSVPubiquitination[2]
527ILAHLHKKCPYSVPFubiquitination[1]
539VPFYPTFKEGMALEDubiquitination[1, 4]
556RMLGYQVKDSKVEQQubiquitination[1, 2]
559GYQVKDSKVEQQDNFubiquitination[1, 2]
568EQQDNFLKRMSGMIRubiquitination[1, 2]
672MGSFIRLKQFLEKCLubiquitination[1, 2]
677RLKQFLEKCLQHKDIubiquitination[1]
682LEKCLQHKDIPVPKGubiquitination[1]
688HKDIPVPKGFLTSSFubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. May be involved in the terminal step of the mRNA transport through the nuclear pore complex (NPC). 
Sequence Annotation
 REGION 1 29 Interaction with NUP155.
 REGION 444 483 Mediates the shuttling between the
 REGION 656 698 Interaction with NUPL2.
 MOD_RES 88 88 Phosphoserine.
 MOD_RES 99 99 Phosphoserine.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Disease mutation; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 698 AA 
Protein Sequence
MPSEGRCWET LKALRSSDKG RLCYYRDWLL RREDVLEECM SLPKLSSYSG WVVEHVLPHM 60
QENQPLSETS PSSTSASALD QPSFVPKSPD ASSAFSPASP ATPNGTKGKD ESQHTESMVL 120
QSSRGIKVEG CVRMYELVHR MKGTEGLRLW QEEQERKVQA LSEMASEQLK RFDEWKELKQ 180
HKEFQDLREV MEKSSREALG HQEKLKAEHR HRAKILNLKL REAEQQRVKQ AEQERLRKEE 240
GQIRLRALYA LQEEMLQLSQ QLDASEQHKA LLKVDLAAFQ TRGNQLCSLI SGIIRASSES 300
SYPTAESQAE AERALREMRD LLMNLGQEIT RACEDKRRQD EEEAQVKLQE AQMQQGPEAH 360
KEPPAPSQGP GGKQNEDLQV KVQDITMQWY QQLQDASMQC VLTFEGLTNS KDSQAKKIKM 420
DLQKAATIPV SQISTIAGSK LKEIFDKIHS LLSGKPVQSG GRSVSVTLNP QGLDFVQYKL 480
AEKFVKQGEE EVASHHEAAF PIAVVASGIW ELHPRVGDLI LAHLHKKCPY SVPFYPTFKE 540
GMALEDYQRM LGYQVKDSKV EQQDNFLKRM SGMIRLYAAI IQLRWPYGNR QEIHPHGLNH 600
GWRWLAQILN MEPLSDVTAT LLFDFLEVCG NALMKQYQVQ FWKMLILIKE DYFPRIEAIT 660
SSGQMGSFIR LKQFLEKCLQ HKDIPVPKGF LTSSFWRS 698 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005643; C:nuclear pore; TAS:ProtInc.
 GO:0006406; P:mRNA export from nucleus; TAS:ProtInc.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IEA:InterPro.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR012476; GLE1. 
Pfam
 PF07817; GLE1 
SMART
  
PROSITE
  
PRINTS