UniProt Accession

 UBP14_RABIT; P40826 

Genbank Protein ID

 L37420 

Genbank Nucleotide ID

 AAA96133.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase 14 

Protein Synonyms/Alias

 Deubiquitinating enzyme 14; Ubiquitin thioesterase 14; Ubiquitin-specific-processing protease 14 

Gene Name

 USP14 

Gene Synonyms/Alias

 TGT 

Created Date

 July 27, 2013 

Organism

 Oryctolagus cuniculus (Rabbit) 

NCBI Taxa ID

 9986 

Lysine Modification

Position	Peptide	Type	References
266	SEEEEVTKGKENQLQ	ubiquitination	[1]
268	EEEVTKGKENQLQLS	ubiquitination	[1]
290	KYLFTGLKLRLQEEI	ubiquitination	[1]
299	RLQEEITKQSPTLQR	ubiquitination	[1]
312	QRNALYIKSSKISRL	ubiquitination	[1]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). 

Sequence Annotation

 DOMAIN 4 80 Ubiquitin-like.
 ACT_SITE 114 114 Nucleophile (By similarity).
 ACT_SITE 434 434 Proton acceptor (By similarity).
 MOD_RES 136 136 Phosphotyrosine (By similarity).
 MOD_RES 143 143 Phosphoserine (By similarity).
 MOD_RES 222 222 Phosphoserine (By similarity).
 MOD_RES 448 448 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Membrane; Phosphoprotein; Protease; Proteasome; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 493 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IEA:Compara.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
 GO:0045202; C:synapse; IEA:Compara.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004866; F:endopeptidase inhibitor activity; IEA:Compara.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:Compara.
 GO:0050920; P:regulation of chemotaxis; IEA:Compara.
 GO:0061136; P:regulation of proteasomal protein catabolic process; IEA:Compara.
 GO:0007268; P:synaptic transmission; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 

Interpro

 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR000626; Ubiquitin.
 IPR019954; Ubiquitin_CS.
 IPR019955; Ubiquitin_supergroup. 

Pfam

 PF00443; UCH 

SMART

 SM00213; UBQ 

PROSITE

 PS00299; UBIQUITIN_1
 PS50053; UBIQUITIN_2
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 

PRINTS