CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002869
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RNA polymerase sigma factor RpoD 
Protein Synonyms/Alias
 Sigma-70 
Gene Name
 rpoD 
Gene Synonyms/Alias
 STM3211 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
253AAQEEILKLSEVFKQacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth (By similarity). 
Sequence Annotation
 DNA_BIND 575 594 H-T-H motif (By similarity).
 REGION 381 451 Sigma-70 factor domain-2 (By similarity).
 REGION 460 536 Sigma-70 factor domain-3 (By similarity).
 REGION 549 602 Sigma-70 factor domain-4 (By similarity).
 MOTIF 405 408 Interaction with polymerase core subunit  
Keyword
 Complete proteome; Cytoplasm; DNA-binding; Reference proteome; Sigma factor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 615 AA 
Protein Sequence
MEQNPQSQLK LLVTRGKEQG YLTYAEVNDH LPEDIVDSDQ IEDIIQMIND MGIQVMEEAP 60
DADDLLLAEN TTSTDEDAEE AAAQVLSSVE SEIGRTTDPV RMYMREMGTV ELLTREGEID 120
IAKRIEDGIN QVQCSVAEYP EAITYLLEQY DRVEAEEARL SDLITGFVDP NAEEEMAPTA 180
THVGSELSQE DLDDDEDEDE EDGDDDAADD DNSIDPELAR EKFAELRAQY VVTRDTIKAK 240
GRSHAAAQEE ILKLSEVFKQ FRLVPKQFDY LVNSMRVMMD RVRTQERLIM KLCVEQCKMP 300
KKNFITLFTG NETSETWFNA AIAMNKPWSE KLHDVAEEVQ RCLQKLRQIE EETGLTIEQV 360
KDINRRMSIG EAKARRAKKE MVEANLRLVI SIAKKYTNRG LQFLDLIQEG NIGLMKAVDK 420
FEYRRGYKFS TYATWWIRQA ITRSIADQAR TIRIPVHMIE TINKLNRISR QMLQEMGREP 480
TPEELAERML MPEDKIRKVL KIAKEPISME TPIGDDEDSH LGDFIEDTTL ELPLDSATTE 540
SLRAATHDVL AGLTAREAKV LRMRFGIDMN TDHTLEEVGK QFDVTRERIR QIEAKALRKL 600
RHPSRSEVLR SFLDD 615 
Gene Ontology
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
 GO:0006352; P:DNA-dependent transcription, initiation; IEA:InterPro. 
Interpro
 IPR014284; RNA_pol_sigma-70_dom.
 IPR000943; RNA_pol_sigma70.
 IPR009042; RNA_pol_sigma70_r1_2.
 IPR007627; RNA_pol_sigma70_r2.
 IPR007624; RNA_pol_sigma70_r3.
 IPR007630; RNA_pol_sigma70_r4.
 IPR007631; RNA_pol_sigma_70_non-ess.
 IPR007127; RNA_pol_sigma_70_r1_1.
 IPR013325; RNA_pol_sigma_r2.
 IPR013324; RNA_pol_sigma_r3_r4.
 IPR012760; RNA_pol_sigma_RpoD_C.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF04546; Sigma70_ner
 PF03979; Sigma70_r1_1
 PF00140; Sigma70_r1_2
 PF04542; Sigma70_r2
 PF04539; Sigma70_r3
 PF04545; Sigma70_r4 
SMART
  
PROSITE
 PS00715; SIGMA70_1
 PS00716; SIGMA70_2 
PRINTS
 PR00046; SIGMA70FCT.