CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004899
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calpastatin 
Protein Synonyms/Alias
 Calpain inhibitor; Sperm BS-17 component 
Gene Name
 CAST 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
500PLLPKESKEQLPPMSacetylation[1]
529PQNASSLKFEDAKLAacetylation[1]
534SLKFEDAKLAAAISEubiquitination[2]
629LDDNGQDKPVKPPTKacetylation[3]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. 
Sequence Annotation
 REPEAT 170 222 Inhibitory domain 1.
 REPEAT 304 356 Inhibitory domain 2.
 REPEAT 446 499 Inhibitory domain 3.
 REPEAT 583 636 Inhibitory domain 4.
 MOD_RES 87 87 Phosphoserine (By similarity).
 MOD_RES 133 133 Phosphoserine.
 MOD_RES 243 243 Phosphoserine.
 MOD_RES 364 364 Phosphoserine.
 MOD_RES 366 366 Phosphoserine.
 MOD_RES 373 373 Phosphoserine.
 MOD_RES 575 575 Phosphoserine.
 MOD_RES 577 577 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Phosphoprotein; Polymorphism; Protease inhibitor; Reference proteome; Repeat; Thiol protease inhibitor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 708 AA 
Protein Sequence
MNPTETKAIP VSQQMEGPHL PNKKKHKKQA VKTEPEKKSQ STKLSVVHEK KSQEGKPKEH 60
TEPKSLPKQA SDTGSNDAHN KKAVSRSAEQ QPSEKSTEPK TKPQDMISAG GESVAGITAI 120
SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK SGMDAALDDL IDTLGGPEET EEENTTYTGP 180
EVSDPMSSTY IEELGKREVT IPPKYRELLA KKEGITGPPA DSSKPIGPDD AIDALSSDFT 240
CGSPTAAGKK TEKEESTEVL KAQSAGTVRS AAPPQEKKRK VEKDTMSDQA LEALSASLGT 300
RQAEPELDLR SIKEVDEAKA KEEKLEKCGE DDETIPSEYR LKPATDKDGK PLLPEPEEKP 360
KPRSESELID ELSEDFDRSE CKEKPSKPTE KTEESKAAAP APVSEAVCRT SMCSIQSAPP 420
EPATLKGTVP DDAVEALADS LGKKEADPED GKPVMDKVKE KAKEEDREKL GEKEETIPPD 480
YRLEEVKDKD GKPLLPKESK EQLPPMSEDF LLDALSEDFS GPQNASSLKF EDAKLAAAIS 540
EVVSQTPAST TQAGAPPRDT SQSDKDLDDA LDKLSDSLGQ RQPDPDENKP MEDKVKEKAK 600
AEHRDKLGER DDTIPPEYRH LLDDNGQDKP VKPPTKKSED SKKPADDQDP IDALSGDLDS 660
CPSTTETSQN TAKDKCKKAA SSSKAPKNGG KAKDSAKTTE ETSKPKDD 708 
Gene Ontology
 GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
 GO:0004866; F:endopeptidase inhibitor activity; TAS:ProtInc.
 GO:2000675; P:negative regulation of type B pancreatic cell apoptotic process; IDA:BHF-UCL. 
Interpro
 IPR026998; Calpastatin.
 IPR001259; Prot_inh_calpain. 
Pfam
 PF00748; Calpain_inhib 
SMART
  
PROSITE
  
PRINTS