CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020221
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 18 
Protein Synonyms/Alias
  
Gene Name
 WDR18 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
263HPEQDAGKVFKGHRNubiquitination[1, 2, 3]
301RLWDVQSKQCIRTVAubiquitination[2, 3]
389VLCSTMEKSVLGGQDubiquitination[3, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 May play a role during development (By similarity). Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. 
Sequence Annotation
 REPEAT 36 75 WD 1.
 REPEAT 78 116 WD 2.
 REPEAT 119 158 WD 3.
 REPEAT 170 211 WD 4.
 REPEAT 213 257 WD 5.
 REPEAT 267 306 WD 6.  
Keyword
 Complete proteome; Cytoplasm; Developmental protein; Nucleus; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 432 AA 
Protein Sequence
MAAPMEVAVC TDSAAPMWSC IVWELHSGAN LLTYRGGQAG PRGLALLNGE YLLAAQLGKN 60
YISAWELQRK DQLQQKIMCP GPVTCLTASP NGLYVLAGVA ESIHLWEVST GNLLVILSRH 120
YQDVSCLQFT GDSSHFISGG KDCLVLVWSL CSVLQADPSR IPAPRHVWSH HALPITDLHC 180
GFGGPLARVA TSSLDQTVKL WEVSSGELLL SVLFDVSIMA VTMDLAEHHM FCGGSEGSIF 240
QVDLFTWPGQ RERSFHPEQD AGKVFKGHRN QVTCLSVSTD GSVLLSGSHD ETVRLWDVQS 300
KQCIRTVALK GPVTNAAILL APVSMLSSDF RPSLPLPHFN KHLLGAEHGD EPRHGGLTLR 360
LGLHQQGSEP SYLDRTEQLQ AVLCSTMEKS VLGGQDQLRV RVTELEDEVR NLRKINRDLF 420
DFSTRFITRP AK 432 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom.
 IPR026987; Wdr18_C_dom. 
Pfam
 PF00400; WD40
 PF14077; WD40_alt 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.