CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019108
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pyrroline-5-carboxylate reductase 2 
Protein Synonyms/Alias
 P5C reductase 2; P5CR 2 
Gene Name
 PYCR2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
47PTVSALRKMGVNLTRubiquitination[1]
107TISSVEKKLMAFQPAubiquitination[1]
116MAFQPAPKVIRCMTNubiquitination[1, 2, 3]
276QSMADQEKISPAALKubiquitination[1]
283KISPAALKKTLLDRVubiquitination[4]
284ISPAALKKTLLDRVKubiquitination[1]
291KTLLDRVKLESPTVSubiquitination[1, 4]
307LTPSSPGKLLTRSLAubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. 
Sequence Annotation
 NP_BIND 6 11 NADP (By similarity).
 NP_BIND 69 72 NADP (By similarity).
 NP_BIND 95 97 NADP (By similarity).
 BINDING 34 34 NADP; via carbonyl oxygen (By
 BINDING 56 56 NADP (By similarity).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 304 304 Phosphoserine.  
Keyword
 Acetylation; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Direct protein sequencing; NADP; Oxidoreductase; Phosphoprotein; Proline biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 320 AA 
Protein Sequence
MSVGFIGAGQ LAYALARGFT AAGILSAHKI IASSPEMNLP TVSALRKMGV NLTRSNKETV 60
KHSDVLFLAV KPHIIPFILD EIGADVQARH IVVSCAAGVT ISSVEKKLMA FQPAPKVIRC 120
MTNTPVVVQE GATVYATGTH ALVEDGQLLE QLMSSVGFCT EVEEDLIDAV TGLSGSGPAY 180
AFMALDALAD GGVKMGLPRR LAIQLGAQAL LGAAKMLLDS EQHPCQLKDN VCSPGGATIH 240
ALHFLESGGF RSLLINAVEA SCIRTRELQS MADQEKISPA ALKKTLLDRV KLESPTVSTL 300
TPSSPGKLLT RSLALGGKKD 320 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004735; F:pyrroline-5-carboxylate reductase activity; IEA:EC.
 GO:0055129; P:L-proline biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR008927; 6-PGluconate_DH_C-like.
 IPR016040; NAD(P)-bd_dom.
 IPR004455; NADP_OxRdtase_F420.
 IPR000304; Pyrroline-COOH_reductase. 
Pfam
 PF03807; F420_oxidored 
SMART
  
PROSITE
 PS00521; P5CR 
PRINTS