CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001582
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-like modifier-activating enzyme ATG7 
Protein Synonyms/Alias
 ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein 
Gene Name
 ATG7 
Gene Synonyms/Alias
 APG7L 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
300AFSPDCPKAVGWEKNubiquitination[1]
306PKAVGWEKNQKGGMGacetylation[2]
309VGWEKNQKGGMGPRMubiquitination[1]
352VPTLDLDKVVSVKCLubiquitination[3]
411EDCLGGGKPKALAAAubiquitination[1]
423AAADRLQKIFPGVNAubiquitination[1]
645PVSLAFDKCTACSSKubiquitination[1]
652KCTACSSKVLDQYERubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3 (By similarity). 
Sequence Annotation
 ACT_SITE 572 572 Glycyl thioester intermediate (By
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Alternative splicing; Autophagy; Complete proteome; Cytoplasm; Polymorphism; Protein transport; Reference proteome; Transport; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 703 AA 
Protein Sequence
MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP 60
ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT 120
ALENPVLLNK FLLLTFADLK KYHFYYWFCY PALCLPESLP LIQGPVGLDQ RFSLKQIEAL 180
ECAYDNLCQT EGVTALPYFL IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY 240
PGWPLRNFLV LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK 300
AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL DKVVSVKCLL 360
LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL YEFEDCLGGG KPKALAAADR 420
LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT LEQARRDVEQ LEQLIESHDV VFLLMDTRES 480
RWLPAVIAAS KRKLVINAAL GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF 540
ANIPGYKLGC YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG 600
GYAIASSSDD RMNEPPTSLG LVPHQIRGFL SRFDNVLPVS LAFDKCTACS SKVLDQYERE 660
GFNFLAKVFN SSHSFLEDLT GLTLLHQETQ AAEIWDMSDD ETI 703 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0019778; F:APG12 activating enzyme activity; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0004839; F:ubiquitin activating enzyme activity; TAS:ProtInc.
 GO:0007628; P:adult walking behavior; IEA:Compara.
 GO:0006914; P:autophagy; IEA:UniProtKB-KW.
 GO:0055013; P:cardiac muscle cell development; IEA:Compara.
 GO:0006520; P:cellular amino acid metabolic process; IEA:Compara.
 GO:0006944; P:cellular membrane fusion; TAS:ProtInc.
 GO:0021955; P:central nervous system neuron axonogenesis; IEA:Compara.
 GO:0021680; P:cerebellar Purkinje cell layer development; IEA:Compara.
 GO:0021987; P:cerebral cortex development; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0050877; P:neurological system process; IEA:Compara.
 GO:0006996; P:organelle organization; IEA:Compara.
 GO:0016239; P:positive regulation of macroautophagy; IMP:BHF-UCL.
 GO:0031401; P:positive regulation of protein modification process; IDA:MGI.
 GO:0009791; P:post-embryonic development; IEA:Compara.
 GO:0030163; P:protein catabolic process; IEA:Compara.
 GO:0006497; P:protein lipidation; IDA:MGI.
 GO:0032446; P:protein modification by small protein conjugation; IEA:Compara.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0021860; P:pyramidal neuron development; IEA:Compara.
 GO:0031396; P:regulation of protein ubiquitination; IEA:Compara.
 GO:0042594; P:response to starvation; IEA:Compara. 
Interpro
 IPR006285; Atg7.
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd. 
Pfam
 PF00899; ThiF 
SMART
  
PROSITE
  
PRINTS