UniProt Accession

 UCHL3_MOUSE; Q9JKB1; Q9EQX7 

Genbank Protein ID

 AF247358; AB033370; BC048481 

Genbank Nucleotide ID

 AAF64193.1; BAB20094.1; AAH48481.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase isozyme L3 

Protein Synonyms/Alias

 UCH-L3; Ubiquitin thioesterase L3 

Gene Name

 Uchl3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
74	TEEEEKIKSQGQDVT	ubiquitination	[1]
121	ESGSTLKKFLEESVS	ubiquitination	[1]
136	MSPEERAKFLENYDA	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome. 

Sequence Annotation

 REGION 8 13 Interaction with ubiquitin (By
 REGION 152 159 Interaction with ubiquitin (By
 REGION 219 224 Interaction with ubiquitin (By
 ACT_SITE 95 95 Nucleophile (By similarity).
 ACT_SITE 169 169 Proton donor (By similarity).
 MOD_RES 130 130 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Hydrolase; Phosphoprotein; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 230 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0008233; F:peptidase activity; IDA:UniProtKB.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0007628; P:adult walking behavior; IGI:MGI.
 GO:0032869; P:cellular response to insulin stimulus; IDA:UniProtKB.
 GO:0042755; P:eating behavior; IGI:MGI.
 GO:0045600; P:positive regulation of fat cell differentiation; IDA:UniProtKB.
 GO:0030163; P:protein catabolic process; IDA:UniProtKB.
 GO:0016579; P:protein deubiquitination; ISA:MGI.
 GO:0060041; P:retina development in camera-type eye; IMP:MGI.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 

Interpro

 IPR001578; Peptidase_C12. 

Pfam

 PF01088; Peptidase_C12 

SMART

  

PROSITE

 PS00140; UCH_1 

PRINTS

 PR00707; UBCTHYDRLASE.