CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019463
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Roundabout homolog 3 
Protein Synonyms/Alias
 Roundabout-like protein 3 
Gene Name
 ROBO3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
1066DSGAKGGKVKLLGKPubiquitination[1]
1068GAKGGKVKLLGKPVQubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing. 
Sequence Annotation
 DOMAIN 64 160 Ig-like C2-type 1.
 DOMAIN 166 253 Ig-like C2-type 2.
 DOMAIN 258 342 Ig-like C2-type 3.
 DOMAIN 347 440 Ig-like C2-type 4.
 DOMAIN 450 531 Ig-like C2-type 5.
 DOMAIN 555 647 Fibronectin type-III 1.
 DOMAIN 669 762 Fibronectin type-III 2.
 DOMAIN 768 863 Fibronectin type-III 3.
 MOD_RES 1263 1263 Phosphoserine.
 CARBOHYD 25 25 N-linked (GlcNAc...) (Potential).
 CARBOHYD 34 34 N-linked (GlcNAc...) (Potential).
 CARBOHYD 41 41 N-linked (GlcNAc...) (Potential).
 CARBOHYD 53 53 N-linked (GlcNAc...) (Potential).
 CARBOHYD 156 156 N-linked (GlcNAc...) (Potential).
 CARBOHYD 410 410 N-linked (GlcNAc...) (Potential).
 CARBOHYD 459 459 N-linked (GlcNAc...) (Potential).
 CARBOHYD 503 503 N-linked (GlcNAc...) (Potential).
 CARBOHYD 784 784 N-linked (GlcNAc...) (Potential).
 CARBOHYD 813 813 N-linked (GlcNAc...) (Potential).
 CARBOHYD 820 820 N-linked (GlcNAc...) (Potential).
 DISULFID 85 143 Potential.
 DISULFID 187 236 Potential.
 DISULFID 279 326 Potential.
 DISULFID 368 424 Potential.
 DISULFID 472 521 Potential.  
Keyword
 Alternative splicing; Chemotaxis; Complete proteome; Developmental protein; Differentiation; Disease mutation; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Neurogenesis; Phosphoprotein; Polymorphism; Receptor; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1386 AA 
Protein Sequence
MLRYLLKTLL QMNLFADSLA GDISNSSELL LGFNSSLAAL NHTLLPPGDP SLNGSRVGPE 60
DAMPRIVEQP PDLLVSRGEP ATLPCRAEGR PRPNIEWYKN GARVATVRED PRAHRLLLPS 120
GALFFPRIVH GRRARPDEGV YTCVARNYLG AAASRNASLE VAVLRDDFRQ SPGNVVVAVG 180
EPAVLECVPP RGHPEPSVSW RKDGARLKEE EGRITIRGGK LMMSHTLKSD AGMYVCVASN 240
MAGERESAAA EVMVLERPSF LRRPVNQVVL ADAPVTFLCE VKGDPPPRLR WRKEDGELPT 300
GRYEIRSDHS LWIGHVSAED EGTYTCVAEN SVGRAEASGS LSVHVPPQLV TQPQDQMAAP 360
GESVAFQCET KGNPPPAIFW QKEGSQVLLF PSQSLQPTGR FSVSPRGQLN ITAVQRGDAG 420
YYVCQAVSVA GSILAKALLE IKGASLDGLP PVILQGPANQ TLVLGSSVWL PCRVTGNPQP 480
SVRWKKDGQW LQGDDLQFKT MANGTLYIAN VQEMDMGFYS CVAKSSTGEA TWSGWLKMRE 540
DWGVSPDPPT EPSSPPGAPS QPVVTEITKN SITLTWKPNP QTGAAVTSYV IEAFSPAAGN 600
TWRTVADGVQ LETHTVSGLQ PNTIYLFLVR AVGAWGLSEP SPVSEPVRTQ DSSPSRPVED 660
PWRGQQGLAE VAVRLQEPIV LGPRTLQVSW TVDGPVQLVQ GFRVSWRVAG PEGGSWTMLD 720
LQSPSQQSTV LRGLPPGTQI QIKVQAQGQE GLGAESLSVT RSIPEEAPSG PPQGVAVALG 780
GDGNSSITVS WEPPLPSQQN GVITEYQIWC LGNESRFHLN RSAAGWARSA MLRGLVPGLL 840
YRTLVAAATS AGVGVPSAPV LVQLPSPPDL EPGLEVGAGL AVRLARVLRE PAFLAGSGAA 900
CGALLLGLCA ALYWRRKQRK ELSHYTASFA YTPAVSFPHS EGLSGASSRP PMGLGPAPYS 960
WLADSWPHPS RSPSAQEPRG SCCPSNPDPD DRYYNEAGIS LYLAQTARGT AAPGEGPVYS 1020
TIDPAGEELQ TFHGGFPQHP SGDLGPWSQY APPEWSQGDS GAKGGKVKLL GKPVQMPSLN 1080
WPEALPPPPP SCELSCLEGP EEELEGSSEP EEWCPPMPER SHLTEPSSSG GCLVTPSRRE 1140
TPSPTPSYGQ QSTATLTPSP PDPPQPPTDM PHLHQMPRRV PLGPSSPLSV SQPMLGIREA 1200
RPAGLGAGPA ASPHLSPSPA PSTASSAPGR TWQGNGEMTP PLQGPRARFR KKPKALPYRR 1260
ENSPGDLPPP PLPPPEEEAS WALELRAAGS MSSLERERSG ERKAVQAVPL AAQRVLHPDE 1320
EAWLPYSRPS FLSRGQGTST CSTAGSNSSR GSSSSRGSRG PGRSRSRSQS RSQSQRPGQK 1380
RREEPR 1386 
Gene Ontology
 GO:0030424; C:axon; IEA:Compara.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0016199; P:axon midline choice point recognition; ISS:UniProtKB.
 GO:0001764; P:neuron migration; IEA:Compara. 
Interpro
 IPR003961; Fibronectin_type3.
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR013098; Ig_I-set.
 IPR003598; Ig_sub2. 
Pfam
 PF00041; fn3
 PF07679; I-set 
SMART
 SM00060; FN3
 SM00408; IGc2 
PROSITE
 PS50853; FN3
 PS50835; IG_LIKE 
PRINTS