UniProt Accession

 OXDA_MOUSE; P18894; Q64465; Q8VCW7 

Genbank Protein ID

 M32299; D10210; D10211; AK134813; BC018377 

Genbank Nucleotide ID

 AAA39367.1; BAA01062.1; BAA01063.1; BAE22296.1; AAH18377.1 

Protein Name

 D-amino-acid oxidase 

Protein Synonyms/Alias

 DAAO; DAMOX; DAO 

Gene Name

 Dao 

Gene Synonyms/Alias

 Dao1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
107	EVPDPFWKNAVLGFR	ubiquitination	[1]
162	GVKLIHRKVESLEEV	ubiquitination	[1]
259	RDHNTIWKSCCKLEP	phosphoglycerylation	[2]
263	TIWKSCCKLEPTLKN	phosphoglycerylation	[2]
269	CKLEPTLKNARIVGE	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237] 

Functional Description

 Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D- amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. 

Sequence Annotation

 NP_BIND 3 17 FAD (By similarity).
 NP_BIND 36 37 FAD (By similarity).
 NP_BIND 43 44 FAD (By similarity).
 NP_BIND 48 50 FAD (By similarity).
 NP_BIND 310 314 FAD (By similarity).
 MOTIF 343 345 Microbody targeting signal.
 BINDING 52 52 Substrate (By similarity).
 BINDING 163 163 FAD; via amide nitrogen and carbonyl
 BINDING 180 180 FAD (By similarity).
 BINDING 215 215 Substrate (By similarity).
 BINDING 226 226 Substrate (By similarity).
 BINDING 281 281 Substrate (By similarity).
 BINDING 311 311 Substrate; via carbonyl oxygen (By
 BINDING 315 315 FAD (By similarity).  

Keyword

 3D-structure; Complete proteome; FAD; Flavoprotein; Oxidoreductase; Peroxisome; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 345 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005741; C:mitochondrial outer membrane; IEA:Compara.
 GO:0005778; C:peroxisomal membrane; IEA:Compara.
 GO:0048037; F:cofactor binding; IDA:UniProtKB.
 GO:0003884; F:D-amino-acid oxidase activity; IDA:UniProtKB.
 GO:0071949; F:FAD binding; IEA:Compara.
 GO:0055130; P:D-alanine catabolic process; IDA:UniProtKB.
 GO:0036088; P:D-serine catabolic process; IEA:Compara.
 GO:0042416; P:dopamine biosynthetic process; IEA:Compara.
 GO:0006551; P:leucine metabolic process; IMP:MGI.
 GO:0006562; P:proline catabolic process; IEA:Compara. 

Interpro

 IPR023209; D-aa_oxidase.
 IPR006181; D-amino_acid_oxidase_CS.
 IPR006076; FAD-dep_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF01266; DAO 

SMART

  

PROSITE

 PS00677; DAO 

PRINTS