UniProt Accession

 CAH3_MOUSE; P16015; Q9ERN8 

Genbank Protein ID

 M27796; AF294988; AF294983; AF294984; AF294985; AF294986; AF294987; BC011129 

Genbank Nucleotide ID

 AAA37355.1; AAG22029.1; AAG22029.1; AAG22029.1; AAG22029.1; AAG22029.1; AAG22029.1; AAH11129.1 

Protein Name

 Carbonic anhydrase 3 

Protein Synonyms/Alias

 Carbonate dehydratase III; Carbonic anhydrase III; CA-III 

Gene Name

 Ca3 

Gene Synonyms/Alias

 Car3 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
3	*****MAKEWGYASH	acetylation	[1]
3	*****MAKEWGYASH	ubiquitination	[2]
24	HELYPIAKGDNQSPI	ubiquitination	[2]
36	SPIELHTKDIKHDPS	acetylation	[1, 3, 4, 5]
36	SPIELHTKDIKHDPS	ubiquitination	[2]
39	ELHTKDIKHDPSLQP	acetylation	[3, 4, 5, 6]
39	ELHTKDIKHDPSLQP	ubiquitination	[2]
57	SYDPGSAKTILNNGK	acetylation	[5]
57	SYDPGSAKTILNNGK	ubiquitination	[2]
64	KTILNNGKTCRVVFD	acetylation	[3, 7]
64	KTILNNGKTCRVVFD	phosphoglycerylation	[8]
64	KTILNNGKTCRVVFD	succinylation	[7]
64	KTILNNGKTCRVVFD	ubiquitination	[2]
126	HLVHWNPKYNTFGEA	acetylation	[3]
126	HLVHWNPKYNTFGEA	ubiquitination	[2]
135	NTFGEALKQPDGIAV	acetylation	[3, 6]
135	NTFGEALKQPDGIAV	ubiquitination	[2]
153	FLKIGREKGEFQILL	acetylation	[3]
153	FLKIGREKGEFQILL	ubiquitination	[2]
165	ILLDALDKIKTKGKE	acetylation	[3]
165	ILLDALDKIKTKGKE	ubiquitination	[2]
171	DKIKTKGKEAPFTHF	acetylation	[4]
171	DKIKTKGKEAPFTHF	ubiquitination	[2]
212	CIVWLLLKEPMTVSS	acetylation	[3, 4]
224	VSSDQMAKLRSLFSS	ubiquitination	[2]
251	WRPPQPVKGRVVRAS	ubiquitination	[2]

Reference

 [1] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [7] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [8] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237] 

Functional Description

 Reversible hydration of carbon dioxide. 

Sequence Annotation

 REGION 64 67 Involved in proton transfer (By
 REGION 198 199 Substrate binding (By similarity).
 ACT_SITE 127 127 By similarity.
 METAL 94 94 Zinc; catalytic.
 METAL 96 96 Zinc; catalytic.
 METAL 119 119 Zinc; catalytic.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 182 182 S-glutathionyl cysteine (By similarity).
 MOD_RES 187 187 S-glutathionyl cysteine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glutathionylation; Lyase; Metal-binding; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 260 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004089; F:carbonate dehydratase activity; IEA:EC.
 GO:0016151; F:nickel cation binding; IDA:MGI.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006730; P:one-carbon metabolic process; IEA:InterPro.
 GO:0006979; P:response to oxidative stress; IEA:Compara. 

Interpro

 IPR001148; Carbonic_anhydrase_a.
 IPR023561; Carbonic_anhydrase_a-class.
 IPR018338; Carbonic_anhydrase_a-class_CS.
 IPR018441; Carbonic_anhydrase_CA3. 

Pfam

 PF00194; Carb_anhydrase 

SMART

 SM01057; Carb_anhydrase 

PROSITE

 PS00162; ALPHA_CA_1
 PS51144; ALPHA_CA_2 

PRINTS