UniProt Accession

 ESA1_YEAST; Q08649; D6W2U6 

Genbank Protein ID

 Z75152; BK006948 

Genbank Nucleotide ID

 CAA99465.1; DAA11012.1 

Protein Name

 Histone acetyltransferase ESA1 

Protein Synonyms/Alias

  

Gene Name

 ESA1 

Gene Synonyms/Alias

 YOR244W; O5257 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
6	**MSHDGKEEPGIAK	acetylation	[1]
13	KEEPGIAKKINSVDD	acetylation	[1]
82	DKEVLYPKLKATDED	acetylation	[2]
96	DNKKQKKKKATNTSE	acetylation	[2]
97	NKKQKKKKATNTSET	acetylation	[2]
135	NLNVQGIKDENISHE	acetylation	[1, 2]
146	ISHEDEIKKLRTSGS	acetylation	[1]
262	SKLFLDHKTLYYDVD	acetylation	[2, 3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [3] MYST protein acetyltransferase activity requires active site lysine autoacetylation.
 Yuan H, Rossetto D, Mellert H, Dang W, Srinivasan M, Johnson J, Hodawadekar S, Ding EC, Speicher K, Abshiru N, Perry R, Wu J, Yang C, Zheng YG, Speicher DW, Thibault P, Verreault A, Johnson FB, Berger SL, Sternglanz R, McMahon SB, Côté J, Marmorstein R.
 EMBO J. 2012 Jan 4;31(1):58-70. [PMID: 22020126] 

Functional Description

 Catalytic component of the NuA4 histone acetyltransferase (HAT) complex which is involved in epigenetic transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4, H3, H2B, H2A and H2A variant H2A.Z. Acetylates histone H4 to form H4K5ac, H4K8ac, H4K12ac and H4K16ac, histone H3 to form H3K14ac, histone H2B to form H2BK16ac, histone H2A to form H2AK4ac and H2AK7ac, and histone variant H2A.Z to form H2A.ZK14ac. Acetylation of histone H4 is essential for DNA double-strand break repair through homologous recombination. Involved in cell cycle progression. Recruitment to promoters depends on H3K4me. 

Sequence Annotation

 DOMAIN 33 86 Chromo.
 REGION 312 318 Acetyl-CoA binding (By similarity).
 MOTIF 245 266 ESA1-RPD3 motif.
 ACT_SITE 262 262
 ACT_SITE 304 304 Nucleophile (By similarity).
 BINDING 307 307 Acetyl-CoA.
 BINDING 342 342 Acetyl-CoA.
 MOD_RES 17 17 Phosphoserine.
 MOD_RES 262 262 N6-acetyllysine; by autocatalysis.  

Keyword

 3D-structure; Acetylation; Activator; Chromatin regulator; Complete proteome; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 445 AA 

Protein Sequence

Gene Ontology

 GO:0032777; C:Piccolo NuA4 histone acetyltransferase complex; IDA:SGD.
 GO:0010485; F:H4 histone acetyltransferase activity; IDA:SGD.
 GO:0034212; F:peptide N-acetyltransferase activity; IMP:SGD.
 GO:0000183; P:chromatin silencing at rDNA; IMP:SGD.
 GO:0006281; P:DNA repair; IDA:SGD.
 GO:0006354; P:DNA-dependent transcription, elongation; IDA:SGD.
 GO:0016239; P:positive regulation of macroautophagy; IMP:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD.
 GO:0051726; P:regulation of cell cycle; IMP:SGD.
 GO:0034401; P:regulation of transcription by chromatin organization; IMP:SGD. 

Interpro

 IPR016181; Acyl_CoA_acyltransferase.
 IPR000953; Chromo_domain/shadow.
 IPR016197; Chromodomain-like.
 IPR002717; MOZ_SAS.
 IPR025995; Tudor-knot. 

Pfam

 PF01853; MOZ_SAS
 PF11717; Tudor-knot 

SMART

 SM00298; CHROMO 

PROSITE

 PS00598; CHROMO_1
 PS50013; CHROMO_2 

PRINTS