CPLM-003926

Genbank Nucleotide ID

Ubiquitin-like protein 4A

Protein Synonyms/Alias

Ubiquitin-like protein GDX

Homo sapiens (Human)

Position	Peptide	Type	References
48	QRLLFKGKALADGKR	acetylation	[1]
54	GKALADGKRLSDYSI	acetylation	[1]
66	YSIGPNSKLNLVVKP	ubiquitination	[2]
72	SKLNLVVKPLEKVLL	ubiquitination	[3]
76	LVVKPLEKVLLEEGE	ubiquitination	[4, 5, 6]
101	QVWQLISKVLARHFS	ubiquitination	[2, 4, 5, 6, 7, 8]
153	EVTETMEKGFSK***	ubiquitination	[4, 5, 6]

[1] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
[2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.

DOMAIN 1 76 Ubiquitin-like.
MOD_RES 90 90 Phosphoserine.

3D-structure; Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0071818; C:BAT3 complex; IDA:UniProtKB.
GO:0019787; F:small conjugating protein ligase activity; TAS:ProtInc.
GO:0006464; P:cellular protein modification process; TAS:ProtInc.
GO:0071816; P:tail-anchored membrane protein insertion into ER membrane; IMP:UniProtKB.
GO:0006810; P:transport; IEA:UniProtKB-KW.

IPR000626; Ubiquitin.
IPR019954; Ubiquitin_CS.
IPR019956; Ubiquitin_subgr.
IPR019955; Ubiquitin_supergroup.

PS00299; UBIQUITIN_1
PS50053; UBIQUITIN_2