UniProt Accession

 CHEY_ECOLI; P0AE67; P06143 

Genbank Protein ID

 K02175; M13463; U00096; AP009048 

Genbank Nucleotide ID

 AAA23577.1; AAA23570.1; AAC74952.1; BAA15698.1 

Protein Name

 Chemotaxis protein CheY 

Protein Synonyms/Alias

  

Gene Name

 cheY 

Gene Synonyms/Alias

 b1882; JW1871 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
4	****MADKELKFLVV	acetylation	[1]
7	*MADKELKFLVVDDF	acetylation	[1, 2]
26	RIVRNLLKELGFNNV	acetylation	[1, 2]
70	MDGLELLKTIRADGA	acetylation	[1]
91	LMVTAEAKKENIIAA	acetylation	[1, 2]
92	MVTAEAKKENIIAAA	acetylation	[1, 2]
109	GASGYVVKPFTAATL	acetylation	[1, 2]
119	TAATLEEKLNKIFEK	acetylation	[1, 2]
122	TLEEKLNKIFEKLGM	acetylation	[1, 2]
126	KLNKIFEKLGM****	acetylation	[2]

Reference

 [1] Acetylation represses the binding of CheY to its target proteins.
 Liarzi O, Barak R, Bronner V, Dines M, Sagi Y, Shainskaya A, Eisenbach M.
 Mol Microbiol. 2010 May;76(4):932-43. [PMID: 20398208]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator. 

Sequence Annotation

 DOMAIN 7 124 Response regulatory.
 METAL 12 12 Magnesium.
 METAL 13 13 Magnesium.
 METAL 57 57 Magnesium.
 METAL 59 59 Magnesium; via carbonyl oxygen.
 MOD_RES 57 57 4-aspartylphosphate.
 MOD_RES 92 92 N6-acetyllysine.
 MOD_RES 109 109 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Chemotaxis; Complete proteome; Cytoplasm; Direct protein sequencing; Flagellar rotation; Magnesium; Metal-binding; Phosphoprotein; Reference proteome; Two-component regulatory system. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 129 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000287; F:magnesium ion binding; IMP:EcoCyc.
 GO:0000156; F:phosphorelay response regulator activity; IEA:InterPro.
 GO:0006935; P:chemotaxis; IMP:EcoCyc.
 GO:0060286; P:flagellar cell motility; TAS:EcoCyc.
 GO:0035556; P:intracellular signal transduction; IEA:GOC.
 GO:0000160; P:phosphorelay signal transduction system; IDA:EcoCyc.
 GO:0006473; P:protein acetylation; IDA:EcoCyc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro. 

Interpro

 IPR011006; CheY-like_superfamily.
 IPR001789; Sig_transdc_resp-reg_receiver. 

Pfam

 PF00072; Response_reg 

SMART

 SM00448; REC 

PROSITE

 PS50110; RESPONSE_REGULATORY 

PRINTS