CPLM-007536

Genbank Nucleotide ID

Enoyl-CoA delta isomerase 1, mitochondrial

Protein Synonyms/Alias

3,2-trans-enoyl-CoA isomerase; Delta(3),Delta(2)-enoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase

Homo sapiens (Human)

Position	Peptide	Type	References
61	GAGVAVMKFKNPPVN	ubiquitination	[1, 2]
89	LEKLENDKSFRGVIL	acetylation	[3]
283	NFVSFISKDSIQKSL	acetylation	[3, 4, 5, 6]
283	NFVSFISKDSIQKSL	ubiquitination	[1, 2, 7, 8]
288	ISKDSIQKSLQMYLE	ubiquitination	[1, 2, 7, 8]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[4] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
[5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
[6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species.

REGION 106 110 Substrate binding.
BINDING 153 153 Substrate; via amide nitrogen.
BINDING 177 177 Substrate.
MOD_RES 89 89 N6-acetyllysine.

3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Fatty acid metabolism; Isomerase; Lipid metabolism; Mitochondrion; Reference proteome; Transit peptide.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
GO:0005759; C:mitochondrial matrix; NAS:UniProtKB.
GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; NAS:UniProtKB.
GO:0006635; P:fatty acid beta-oxidation; NAS:UniProtKB.

IPR001753; Crotonase_core_superfam.
IPR018376; Enoyl-CoA_hyd/isom_CS.

PS00166; ENOYL_COA_HYDRATASE