CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018323
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kelch repeat and BTB domain-containing protein 7 
Protein Synonyms/Alias
  
Gene Name
 KBTBD7 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
178TNCTAILKFADAFDHubiquitination[1]
187ADAFDHHKLRSQAQSubiquitination[1]
201SYIAHNFKQLSRMGSubiquitination[1, 2, 3]
252QWLEAAAKERGPSAAubiquitination[1]
263PSAAEVFKCVRWMHFubiquitination[1]
284YLEGLLTKPIVKKYCubiquitination[1]
289LTKPIVKKYCLDVIEubiquitination[1]
309RYGDLLYKSLVPVPNubiquitination[1]
403YLAAQPRKDLWVYKPubiquitination[1, 2, 3]
409RKDLWVYKPAQNSWQubiquitination[1]
452PITGVKLKEVECYSVubiquitination[1, 4, 5]
511WLNCASLKRSDFQEAubiquitination[1, 6]
535ICDIPVMKVYNPARGubiquitination[6]
567QIVNHDQKLLLITSTubiquitination[1]
579TSTTPQWKKNRVTVYubiquitination[1, 2, 3, 4, 5]
580STTPQWKKNRVTVYEubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
 DOMAIN 63 138 BTB.
 REPEAT 386 435 Kelch 1.
 REPEAT 436 484 Kelch 2.
 REPEAT 486 523 Kelch 3.
 REPEAT 524 564 Kelch 4.
 REPEAT 567 616 Kelch 5.  
Keyword
 Complete proteome; Kelch repeat; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 684 AA 
Protein Sequence
MQSREDVPRS RRLASPRGGR RPKRISKPSV SAFFTGPEEL KDTAHSAALL AQLKSFYDAR 60
LLCDVTIEVV TPGSGPGTGR LFSCNRNVLA AACPYFKSMF TGGMYESQQA SVTMHDVDAE 120
SFEVLVDYCY TGRVSLSEAN VQRLYAASDM LQLEYVREAC ASFLARRLDL TNCTAILKFA 180
DAFDHHKLRS QAQSYIAHNF KQLSRMGSIR EETLADLTLA QLLAVLRLDS LDIESERTVC 240
HVAVQWLEAA AKERGPSAAE VFKCVRWMHF TEEDQDYLEG LLTKPIVKKY CLDVIEGALQ 300
MRYGDLLYKS LVPVPNSSSS SSSSNSLVSA AENPPQRLGM CAKEMVIFFG HPRDPFLCYD 360
PYSGDIYTMP SPLTSFAHTK TVTSSAVCVS PDHDIYLAAQ PRKDLWVYKP AQNSWQQLAD 420
RLLCREGMDV AYLNGYIYIL GGRDPITGVK LKEVECYSVQ RNQWALVAPV PHSFYSFELI 480
VVQNYLYAVN SKRMLCYDPS HNMWLNCASL KRSDFQEACV FNDEIYCICD IPVMKVYNPA 540
RGEWRRISNI PLDSETHNYQ IVNHDQKLLL ITSTTPQWKK NRVTVYEYDT REDQWINIGT 600
MLGLLQFDSG FICLCARVYP SCLEPGQSFI TEEDDARSES STEWDLDGFS ELDSESGSSS 660
SFSDDEVWVQ VAPQRNAQDQ QGSL 684 
Gene Ontology
  
Interpro
 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR017096; Kelch-like_gigaxonin.
 IPR015915; Kelch-typ_b-propeller.
 IPR006652; Kelch_1. 
Pfam
 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 
SMART
 SM00875; BACK
 SM00225; BTB 
PROSITE
 PS50097; BTB 
PRINTS