CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008607
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 
Protein Synonyms/Alias
 Helicase JA1 
Gene Name
 PRP43 
Gene Synonyms/Alias
 YGL120C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
662GFFMQVAKKRSGAKGmethylation[1]
Reference
 [1] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074] 
Functional Description
 Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. 
Sequence Annotation
 DOMAIN 103 268 Helicase ATP-binding.
 DOMAIN 293 473 Helicase C-terminal.
 NP_BIND 116 123 ATP (By similarity).
 MOTIF 215 218 DEAH box.
 MOD_RES 8 8 Phosphoserine.
 MOD_RES 9 9 Phosphoserine.  
Keyword
 3D-structure; ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 767 AA 
Protein Sequence
MGSKRRFSSE HPDPVETSIP EQAAEIAEEL SKQHPLPSEE PLVHHDAGEF KGLQRHHTSA 60
EEAQKLEDGK INPFTGREFT PKYVDILKIR RELPVHAQRD EFLKLYQNNQ IMVFVGETGS 120
GKTTQIPQFV LFDEMPHLEN TQVACTQPRR VAAMSVAQRV AEEMDVKLGE EVGYSIRFEN 180
KTSNKTILKY MTDGMLLREA MEDHDLSRYS CIILDEAHER TLATDILMGL LKQVVKRRPD 240
LKIIIMSATL DAEKFQRYFN DAPLLAVPGR TYPVELYYTP EFQRDYLDSA IRTVLQIHAT 300
EEAGDILLFL TGEDEIEDAV RKISLEGDQL VREEGCGPLS VYPLYGSLPP HQQQRIFEPA 360
PESHNGRPGR KVVISTNIAE TSLTIDGIVY VVDPGFSKQK VYNPRIRVES LLVSPISKAS 420
AQQRAGRAGR TRPGKCFRLY TEEAFQKELI EQSYPEILRS NLSSTVLELK KLGIDDLVHF 480
DFMDPPAPET MMRALEELNY LACLDDEGNL TPLGRLASQF PLDPMLAVML IGSFEFQCSQ 540
EILTIVAMLS VPNVFIRPTK DKKRADDAKN IFAHPDGDHI TLLNVYHAFK SDEAYEYGIH 600
KWCRDHYLNY RSLSAADNIR SQLERLMNRY NLELNTTDYE SPKYFDNIRK ALASGFFMQV 660
AKKRSGAKGY ITVKDNQDVL IHPSTVLGHD AEWVIYNEFV LTSKNYIRTV TSVRPEWLIE 720
IAPAYYDLSN FQKGDVKLSL ERIKEKVDRL NELKQGKNKK KSKHSKK 767 
Gene Ontology
 GO:0030686; C:90S preribosome; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0071014; C:post-mRNA release spliceosomal complex; IMP:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; IDA:SGD.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0000466; P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0000390; P:spliceosomal complex disassembly; IDA:SGD. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind 
SMART
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS